Jiang Mei, Xu Yicen, Wang Liqiang, Liu Jingting, Yu Jie, Chen Haimei
Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, P. R. China.
College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
Mitochondrial DNA B Resour. 2020 Jul 25;5(3):2948-2953. doi: 10.1080/23802359.2020.1791001.
L. is an important commercial and medicinal plant. In this study, we reported the complete chloroplast genome sequence of . The genome has a circular structure of 152,076 bp containing a large single-copy region (LSC) of 83,914 bp, a small copy region (SSC) of 18,064 bp, and two inverted repeats (IR) of 25,049 bp by each. It harbors 111 unique genes, including 79 protein-coding genes, 4 ribosomal RNA genes, and 28 transfer RNA genes. A total of 41 microsatellite, 20 tandem, and 37 interspersed repeats were detected in the genome. The phylogenomic analysis shows that is a single phylogenetic cluster. The complete chloroplast genome of lays the foundation for the phylogenetic, evolutionary, and conservation studies of the genus . Furthermore, the intergenic region of B-L was variable among the species . This suggests that this region might be a mutation hotspot and will be useful for phylogenetic study and the development of molecular markers. At last, we systematically identified the RNA editing sites in the chloroplast genome of based on the transcriptome downloaded from the SRA database. This study identified the characteristics of the chloroplast genome, SNPs, and RNA editing sites, which will facilitate species identification and phylogenetic analysis within .
L.是一种重要的商业和药用植物。在本研究中,我们报道了L.的完整叶绿体基因组序列。该基因组具有152,076 bp的环状结构,包含一个83,914 bp的大单拷贝区域(LSC)、一个18,064 bp的小单拷贝区域(SSC)以及两个各为25,049 bp的反向重复序列(IR)。它含有111个独特基因,包括79个蛋白质编码基因、4个核糖体RNA基因和28个转运RNA基因。在基因组中总共检测到41个微卫星、20个串联重复和37个散布重复序列。系统发育基因组学分析表明L.是一个单一的系统发育簇。L.的完整叶绿体基因组为该属的系统发育、进化和保护研究奠定了基础。此外,B - L的基因间区域在各物种间存在差异。这表明该区域可能是一个突变热点,将有助于系统发育研究和分子标记的开发。最后,我们基于从SRA数据库下载的转录组,系统地鉴定了L.叶绿体基因组中的RNA编辑位点。本研究确定了L.叶绿体基因组的特征、单核苷酸多态性(SNP)和RNA编辑位点,这将有助于L.属内的物种鉴定和系统发育分析。