Ahmed Md Sagir, Datta Sujan Kumar, Saha Tonmoy, Hossain Zarif
Department of Zoology University of Dhaka Dhaka Bangladesh.
Department of Zoology Jagannath University Dhaka Bangladesh.
Ecol Evol. 2021 Apr 7;11(9):3696-3709. doi: 10.1002/ece3.7355. eCollection 2021 May.
This study describes the molecular characterization of marine and coastal fishes of Bangladesh based on the mitochondrial cytochrome c oxidase subunit I (COI) gene as a marker. A total of 376 mitochondrial COI barcode sequences were obtained from 185 species belonging to 146 genera, 74 families, 21 orders, and two classes of fishes. The mean length of the sequences was 652 base pairs. In Elasmobranchii (Sharks and rays), the average Kimura two parameter (K2P) distances within species, genera, families, and orders were 1.20%, 6.07%, 11.08%, and 14.68%, respectively, and for Actinopterygii, the average K2P distances within species, genera, families, and orders were 0.40%, 6.36%, 14.10%, and 24.07%, respectively. The mean interspecies distance was 16-fold higher than the mean intraspecies distance. The K2P neighbor-joining (NJ) trees based on the sequences generally clustered species in accordance with their taxonomic position. A total of 21 species were newly recorded in Bangladesh. High efficiency and fidelity in species identification and discrimination were demonstrated in the present study by DNA barcoding, and we conclude that COI sequencing can be used as an authentic identification marker for Bangladesh marine fish species.
本研究以线粒体细胞色素c氧化酶亚基I（COI）基因为标记，描述了孟加拉国海洋和沿海鱼类的分子特征。共获得了来自185个物种的376条线粒体COI条形码序列，这些物种分属于146个属、74个科、21个目以及两类鱼。序列的平均长度为652个碱基对。在板鳃亚纲（鲨鱼和鳐鱼）中，种内、属内、科内和目内的平均Kimura双参数（K2P）距离分别为1.20%、6.07%、11.08%和14.68%，而在辐鳍鱼纲中，种内、属内、科内和目内的平均K2P距离分别为0.40%、6.36%、14.10%和24.07%。种间平均距离比种内平均距离高16倍。基于这些序列构建的K2P邻接法（NJ）树通常根据物种的分类地位将它们聚类。孟加拉国新记录了21个物种。本研究通过DNA条形码技术在物种鉴定和区分方面展示了高效性和准确性，我们得出结论，COI测序可作为孟加拉国海洋鱼类物种的可靠鉴定标记。
