Ruang-Areerate Panthita, Shearman Jeremy, Kongkachana Wasitthee, Jomchai Nukoon, Yoocha Thippawan, U-Thoomporn Sonicha, Narong Nattapol, Sheedy John Robert, Mekiyanon Supat, Pootakham Wirulda, Tangphatsornruang Sithichoke
National Omics Center, National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand.
Chia Tai Company Limited, Phra Khanong District, Bangkok, Thailand.
Mitochondrial DNA B Resour. 2020 Aug 26;5(3):3208-3209. doi: 10.1080/23802359.2020.1810165.
Based on PacBio assembly, we report the first complete mitochondrial genome of (460,333 bp) containing nine large chloroplast-derived sequences (1.9-17.3 kb) across the mitogenome. The base composition of the mitogenome in descending order is A: 28.02%, C: 22.04%, G: 21.83% and T: 28.10%, and the G + C content is 43.87%. There are 63 mitochondrial genes including 40 protein-coding genes, 3 rRNA genes and 20 tRNA genes. Additionally, a total of 288 repeats ranging from 31 to 5,301 bp were identified, accounting for 5.7% of the mitogenome. Two large direct repeats (5,301 and 405 bp) within the mitogenome were found for the formation of four subgenomic molecules. A phylogenetic analysis showed that was closely related to other species in Cucurbiaceae. This mitogenome provides useful genetic information for evolutionary studies.
基于PacBio组装,我们报道了首个完整的线粒体基因组(460,333 bp),其在有丝分裂基因组中包含9个大的叶绿体衍生序列(1.9 - 17.3 kb)。有丝分裂基因组的碱基组成按降序排列为:A:28.02%,C:22.04%,G:21.83%,T:28.10%,G + C含量为43.87%。有63个线粒体基因,包括40个蛋白质编码基因、3个rRNA基因和20个tRNA基因。此外,共鉴定出288个长度从31到5301 bp的重复序列,占线粒体基因组的5.7%。在有丝分裂基因组中发现了两个大的正向重复序列(5301和405 bp),用于形成四个亚基因组分子。系统发育分析表明,[物种名称未给出]与葫芦科中的其他物种密切相关。这个有丝分裂基因组为进化研究提供了有用的遗传信息。