Kundu Shantanu, Lalremsanga Hmar Tlawmte, Biakzuala Lal, Chandra Kailash, Kumar Vikas
Molecular Systematics Division, Zoological Survey of India, Centre for DNA Taxonomy, Kolkata, India.
Developmental Biology and Herpetology Laboratory, Department of Zoology, Mizoram University, Aizawl, India.
Mitochondrial DNA B Resour. 2020 Aug 26;5(3):3229-3233. doi: 10.1080/23802359.2020.1810147.
The DNA barcode data of Asian Glass Lizard, , is limited in the global database, especially from India. The present study aimed to generate a barcode sequence of morphologically identified from the Mizoram state in northeast India and compared with other Anguidae species. The studied species showed monophyletic clustering in the Bayesian analysis (BA) phylogeny with strong posterior probability support and also discriminated sufficient Kimura 2 parameter genetic distances. The barcode data of revealed high intra-species genetic variability and formed two clusters in BA phylogeny. The Templeton, Crandall, and Sing network also depicted four different haplotypes within the barcode sequences of . The DNA sequences generated from northeast India showed 6.5-6.6% and 7.3% genetic distances with the sequences generated from Yunnan Province and Tibetan Plateau, respectively. Considering the high genetic distances, multiple clustering, and distinct haplotypes, the present study assumed the presence of possible cryptic diversity of in the Indochina sub-region and a distinct population in northeast India. We recommended the generation of more DNA information from different localities to elucidate the actual diversity of within the known range distribution.
亚洲玻璃蜥蜴(学名:Ophisaurus ventralis)的DNA条形码数据在全球数据库中有限,尤其是来自印度的相关数据。本研究旨在从印度东北部米佐拉姆邦形态学鉴定的Ophisaurus ventralis中生成条形码序列,并与其他蜥蜴科物种进行比较。在贝叶斯分析(BA)系统发育中,所研究的物种显示出单系聚类,具有很强的后验概率支持,并且也区分出了足够的Kimura 2参数遗传距离。Ophisaurus ventralis的条形码数据显示出较高的种内遗传变异性,并在BA系统发育中形成了两个聚类。Templeton、Crandall和Sing网络也在Ophisaurus ventralis的条形码序列中描绘了四种不同的单倍型。从印度东北部生成的DNA序列与从云南省和青藏高原生成的序列分别显示出6.5 - 6.6%和7.3%的遗传距离。考虑到高遗传距离、多重聚类和不同的单倍型,本研究推测在印度支那次区域可能存在Ophisaurus ventralis的潜在隐存多样性,以及在印度东北部存在一个独特的种群。我们建议从不同地点生成更多的DNA信息,以阐明已知分布范围内Ophisaurus ventralis的实际多样性。