甘蔗（品种杂交种）15a - 53的线粒体全基因组及系统发育分析
The complete mitochondrial genome and phylogenetic analysis of sugarcane ( spp. hybrids) line 15a-53.
作者信息
Liu Xiaolan, Xu Liping, Que Youxiong, Ling Hui, Yin Ze, Liu Ying, Zhou Dinggang
机构信息
Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan, PR China.
Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture/National Engineering Research Center for Sugarcane, Ministry of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, PR China.
出版信息
Mitochondrial DNA B Resour. 2020 Sep 21;5(3):3389-3391. doi: 10.1080/23802359.2020.1820395.
The complete mitogenome of spp. hybrid 15a-53 was determined in this study, which contains two distinct circular chromosomes, Chromosome 1 and 2. The length of Chromosome 1 is 300,848 bp with the GC content of 43.93%, while Chromosome 2 is 144,713 bp in length with the GC content of 43.57%. In Chromosome 1, 7.14% of the genome (21,468 nucleotides) is coding DNA and 92.86% (279,380 nucleotides) are intergenic region, while in Chromosome 2, 8.20% of genome (11,865 nucleotides) are coding DNA and 91.80% (132,848 nucleotides) are intergenic region. Chromosome 1 contains 20 protein-coding genes (three genes, three genes, two genes, one gene, one gene, six genes, and four genes), and 21 non-coding genes (15 tRNA and six rRNAs), while in Chromosome 2, there are 13 protein-coding genes (four genes, three genes, two genes, one gene, one gene, one gene, and one gene) and five tRNA genes. Maximum Likelihood phylogenetic analysis indicated that 15a-53 is close to spp. hybrid ROC22, spp. hybrid FN15 and Khon Kaen 3. The complete mitochondrial genome herein will provide useful sequence information for phylogenetic and evolutionary studies for Saccharum and Poaceae.
本研究确定了 spp. 杂交种 15a - 53 的完整线粒体基因组，其包含两条不同的环状染色体，即染色体 1 和染色体 2。染色体 1 的长度为 300,848 碱基对，GC 含量为 43.93%，而染色体 2 的长度为 144,713 碱基对，GC 含量为 43.57%。在染色体 1 中，基因组的 7.14%（21,468 个核苷酸）是编码 DNA，92.86%（279,380 个核苷酸）是基因间隔区；而在染色体 2 中，基因组的 8.20%（11,865 个核苷酸）是编码 DNA，91.80%（132,848 个核苷酸）是基因间隔区。染色体 1 包含 20 个蛋白质编码基因（三个 基因、三个 基因、两个 基因、一个 基因、一个 基因、六个 基因和四个 基因）以及 21 个非编码基因（15 个 tRNA 和 6 个 rRNA），而在染色体 2 中，有 13 个蛋白质编码基因（四个 基因、三个 基因、两个 基因、一个 基因、一个 基因、一个 基因和一个 基因）以及 5 个 tRNA 基因。最大似然系统发育分析表明，15a - 53 与 spp. 杂交种 ROC22、spp. 杂交种 FN15 和 Khon Kaen 3 亲缘关系较近。本文的完整线粒体基因组将为甘蔗属和禾本科的系统发育及进化研究提供有用的序列信息。
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