Liu Xiaolan, Yin Ze, Liu Ying, Li Zhu, Yan Mingli, Que Youxiong, Xu Liping, Zhou Dinggang
Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan, China.
Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture/National Engineering Research Center for Sugarcane, Ministry of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China.
Mitochondrial DNA B Resour. 2020 May 27;5(3):2163-2165. doi: 10.1080/23802359.2020.1768926. eCollection 2020.
The complete mitogenome of spp. hybrid FN15 was successfully sequenced. It contains two distinct circular chromosomes, Chromosome 1 and Chromosome 2. The former is 301,533 bp in length with the GC content of 43.90%, and 7.12% of genome (21,468 nucleotides) are coding DNA while 92.88% of genome (280,065 nucleotides) are intergenic region. The latter is 144,744 bp in length with the GC content of 43.57%, and 8.20% of genome (11,865 nucleotides) are coding DNA and 91.80% of genome (132,879 nucleotides) are intergenic region. Besides, Chromosome 1 contains 22 protein-coding genes (four genes, three genes, three genes, one gene, one gene, six genes and four genes), and 21 non-coding genes (15 tRNA and six rRNAs), whereas in Chromosome 2, there are 13 protein-coding genes (two genes, one gene, one gene, one gene, one gene, four genes and three genes) and five tRNA genes. Maximum Likelihood phylogenetic analysis demonstrated that FN15 is close with spp. hybrid ROC22, Khon Kaen 3 and species. This complete mitochondrial genome will provide essential DNA molecular data for further phylogenetic and evolutionary analysis for .
某物种杂交种FN15的完整线粒体基因组已成功测序。它包含两个不同的环状染色体,即染色体1和染色体2。前者长度为301,533 bp,GC含量为43.90%,基因组的7.12%(21,468个核苷酸)为编码DNA,而基因组的92.88%(280,065个核苷酸)为基因间隔区。后者长度为144,744 bp,GC含量为43.57%,基因组的8.20%(11,865个核苷酸)为编码DNA,91.80%(132,879个核苷酸)为基因间隔区。此外,染色体1包含22个蛋白质编码基因(四个[具体基因名称1]基因、三个[具体基因名称2]基因、三个[具体基因名称3]基因、一个[具体基因名称4]基因、一个[具体基因名称5]基因、六个[具体基因名称6]基因和四个[具体基因名称7]基因),以及21个非编码基因(15个tRNA和6个rRNA),而在染色体2中,有13个蛋白质编码基因(两个[具体基因名称8]基因、一个[具体基因名称9]基因、一个[具体基因名称10]基因、一个[具体基因名称11]基因、一个[具体基因名称12]基因、四个[具体基因名称13]基因和三个[具体基因名称14]基因)和5个tRNA基因。最大似然系统发育分析表明,FN15与某物种杂交种ROC22、孔敬3和某物种关系密切。这个完整的线粒体基因组将为该物种进一步的系统发育和进化分析提供重要的DNA分子数据。