Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Department of Chemistry and Biochemistry at UCLA, Los Angeles, CA 90095, USA.
STAR Protoc. 2020 Dec 30;2(1):100243. doi: 10.1016/j.xpro.2020.100243. eCollection 2021 Mar 19.
Mitochondria play a central role in lipid metabolism and can bind to lipid droplets. However, the role and functional specialization of the population of peridroplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describe an approach to isolate intact PDMs from murine brown adipose tissue based on their adherence to lipid droplets. PDMs isolated using our approach can be used to study their specialized function by respirometry. For complete information on the use and execution of this protocol, please refer to Benador et al. (2018).
线粒体在脂质代谢中发挥核心作用，并可与脂滴结合。然而，直到最近才开发出分离功能完整的脂滴相关线粒体（PDM）的方法，因此，PDM 群体的作用和功能特化仍不清楚。在此，我们描述了一种基于与脂滴的黏附性从鼠褐色脂肪组织中分离完整 PDM 的方法。使用我们的方法分离的 PDM 可通过呼吸测量法来研究其特化功能。如需了解本方案的使用和实施的完整信息，请参考 Benador 等人（2018 年）。
