Pais Ana Karolina Leite, Silva Jessica Rodrigues da, Santos Leandro Victor Silva Dos, Albuquerque Greecy Mirian Rodrigues, Farias Antonio Roberto Gomes de, Silva Junior Wilson José, Balbino Valdir de Queiroz, Silva Adriano Márcio Freire, Gama Marco Aurélio Siqueira da, Souza Elineide Barbosa de
Universidade Federal Rural de Pernambuco (UFRPE), Departamento de Agronomia, Recife, PE, Brazil.
Instituto Federal de Educação, Ciência e Tecnologia do Piauí (IFPI), Piauí, PI, Brazil.
Genet Mol Biol. 2021 Jan 8;44(1):e20200172. doi: 10.1590/1678-4685-GMB-2020-0172. eCollection 2021.
Banana vascular wilt or Moko is a disease caused by Ralstonia solanacearum. This study aimed to sequence, assemble, annotate, and compare the genomes of R. solanacearum Moko ecotypes of different sequevar strains from Brazil. Average nucleotide identity analyses demonstrated a high correlation (> 96%) between the genome sequences of strains CCRMRs277 (sequevar IIA-24), CCRMRs287 (IIB-4), CCRMRs304 (IIA-24), and CCRMRsB7 (IIB-25), which were grouped into phylotypes IIA and IIB. The number of coding sequences present in chromosomes and megaplasmids varied from 3,070 to 3,521 and 1,669 to 1,750, respectively. Pangenome analysis identified 3,378 clusters in the chromosomes, of which 2,604 were shared by all four analyzed genomes and 2,580 were single copies. In megaplasmids, 1,834 clusters were identified, of which 1,005 were shared by all four genomes and 992 were identified as single copies. Strains CCRMRsB7 and CCRMRs287 differed from the others by having unique clusters in both their chromosomes and megaplasmids, and CCRMRsB7 possessed the largest genome among all Moko ecotype strains sequenced to date. Therefore, the genomic information obtained in this study provides a theoretical basis for the identification, characterization, and phylogenetic analysis of R. solanacearum Moko ecotypes.
香蕉维管束枯萎病或莫科病是由青枯雷尔氏菌引起的一种疾病。本研究旨在对来自巴西的不同序列变种菌株的青枯雷尔氏菌莫科生态型的基因组进行测序、组装、注释和比较。平均核苷酸同一性分析表明，菌株CCRMRs277（序列变种IIA - 24）、CCRMRs287（IIB - 4）、CCRMRs304（IIA - 24）和CCRMRsB7（IIB - 25）的基因组序列之间具有高度相关性（> 96%），这些菌株被归为IIA和IIB系统发育型。染色体和大质粒中存在的编码序列数量分别从3070到3521以及1669到1750不等。泛基因组分析在染色体中鉴定出3378个聚类，其中2604个为所有四个分析基因组所共有，2580个为单拷贝。在大质粒中，鉴定出1834个聚类，其中1005个为所有四个基因组所共有，992个被鉴定为单拷贝。菌株CCRMRsB7和CCRMRs287与其他菌株的不同之处在于它们的染色体和大质粒中都有独特的聚类，并且CCRMRsB7在迄今测序的所有莫科生态型菌株中拥有最大的基因组。因此，本研究获得的基因组信息为青枯雷尔氏菌莫科生态型的鉴定、特征描述和系统发育分析提供了理论基础。
