Shekhar Sudhanshu, Karipott Salil S, Guldberg Robert E, Ong Keat G
Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, Eugene, Oregon, USA.
Biotechnol Bioeng. 2021 Jun;118(6):2380-2385. doi: 10.1002/bit.27680. Epub 2021 Apr 2.
Magnetoelastic (ME) sensors, which can be remotely activated via magnetic fields, are an excellent choice for wireless monitoring of biological parameters due to their ability to be scaled into different sizes and have their surface functionalized for chemical or biological sensing. In this study, we present the application of a commercially available ME material (Metglas 2826 MB) to develop a sensor system that can monitor the attachment of anchorage-dependent mammalian cells in two-dimensional in vitro cell cultures. Results obtained with the developed sensors and detection system correlated with microscopic image analysis of cell quantification, which showed a linear relationship between the sensor response and attached fibroblast cells on the sensor surface. It was also revealed that the developed ME sensor system is capable of providing temporal profiles of cell growth corresponding to different stages of cell attachment and proliferation in real-time.
磁弹性（ME）传感器可通过磁场进行远程激活，由于其能够被制作成不同尺寸并对其表面进行功能化处理以用于化学或生物传感，因此是无线监测生物参数的理想选择。在本研究中，我们展示了一种市售ME材料（Metglas 2826 MB）在开发传感器系统中的应用，该系统能够监测二维体外细胞培养中贴壁依赖型哺乳动物细胞的附着情况。使用所开发的传感器和检测系统获得的结果与细胞定量的显微镜图像分析相关，这表明传感器响应与传感器表面附着的成纤维细胞之间存在线性关系。研究还表明，所开发的ME传感器系统能够实时提供与细胞附着和增殖不同阶段相对应的细胞生长时间曲线。
