Mangiferin potentiates the activity of antifungal agents against fluconazole-resistant spp.

To evaluate the antifungal activity of mangiferin against spp. resistant to fluconazole. The antifungal activity of mangiferin was assessed using broth microdilution and its interaction with azoles and amphotericin B was evaluated by checkerboard. The activity of mangiferin against spp. biofilms was assessed using the MTT colorimetric assay and its possible mechanism of action was evaluated using flow cytometry. Mangiferin showed activity against and resistant to fluconazole and showed synergism with azoles and amphotericin B. Mangiferin increased the activity of antifungals against biofilms and caused depolarization of the mitochondrial membrane and externalization of phosphatidylserine, suggesting apoptosis. mangiferin combined with antifungals has potential against spp.