Photobiology Applied to Health, Research & Development Institute, Univap Universidade do Vale do Paraíba, São José dos Campos, São Paulo, Brazil.
Future Microbiol. 2021 Jan;16(2):95-106. doi: 10.2217/fmb-2020-0019. Epub 2021 Jan 18.
This study aimed to evaluate the effect of photodynamic therapy (PDT) with Photogem in promastigotes of and , and in infected macrophages. The following parameters were analyzed: Photogem internalization, mitochondrial activity, viability, tubulin marking and morphological alterations in promastigotes and viability in infected macrophages. Photogem accumulated in the cytosol and adhered to the flagellum. Changes were observed in the mitochondrial activity in groups maintained in the dark, with no viability alteration. After PDT, viability decreased up to 80%, and morphology was affected. The results point out that PDT with Photogem can reduce parasite and macrophage viability.
本研究旨在评估 Photogem 光动力疗法（PDT）对 和 前鞭毛体以及感染巨噬细胞的影响。分析了以下参数： Photogem 摄取、线粒体活性、活力、微管标记和前鞭毛体形态改变以及感染巨噬细胞的活力。 Photogem 在细胞质中积累并附着在鞭毛上。在黑暗中培养的组中观察到线粒体活性发生变化，但活力没有改变。PDT 后，寄生虫和巨噬细胞的活力下降了 80%，形态也受到影响。研究结果表明，Photogem PDT 可以降低寄生虫和巨噬细胞的活力。
