Silva Emanoel Pedro de Oliveira, Mittmann Josane, Ferreira Vitória Tonini Porto, Cardoso Maria Angélica Gargione, Beltrame Milton
Laboratório de Síntese Orgânica, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, 2911 Shishima Hifumi Avenue, 12244-000, São José dos Campos, SP, Brazil.
Lasers Med Sci. 2015 Jan;30(1):347-54. doi: 10.1007/s10103-014-1665-6. Epub 2014 Oct 7.
This study investigated the photoactivity of four zinc phthalocyanines (PcZns) on a murine macrophage cell line infected with Leishmania amazonensis or Leishmania braziliensis. Infected and uninfected cells were incubated with PcZns at different concentrations (1-10 μM) for 3 h and then exposed to an LED device in continuous wave mode at 660 nm with a fluency of 50 J/cm(2) (25 mV). Enzymatic activity was determined by MTT assay 24 h after light treatment. The results demonstrated that all PcZns exhibited high photoactivity, particularly when used at 10 μM. The photodynamic effects were different for uninfected cells versus parasite-infected cells and among the four PcZns. Uninfected cells were more sensitive to photoactivity than infected cells. Although PcZns photodynamic therapy provided promising results, further studies are necessary to better understand its mechanism of action in the treatment of leishmaniasis.
本研究调查了四种锌酞菁（PcZns）对感染亚马逊利什曼原虫或巴西利什曼原虫的小鼠巨噬细胞系的光活性。将感染和未感染的细胞与不同浓度（1 - 10 μM）的PcZns孵育3小时，然后以连续波模式暴露于660 nm的LED装置下，通量为50 J/cm(2)（25 mV）。光照处理24小时后，通过MTT法测定酶活性。结果表明，所有的PcZns都表现出高光活性，尤其是在10 μM浓度下使用时。未感染细胞与寄生虫感染细胞以及四种PcZns之间的光动力效应有所不同。未感染细胞比感染细胞对光活性更敏感。尽管PcZns光动力疗法取得了有前景的结果，但仍需要进一步研究以更好地理解其在治疗利什曼病中的作用机制。
