Research and Development Institute-IPD, Vale do Paraíba University-UNIVAP, Av. Shishima Hifumi, 2911, São José Dos Campos, SP, CEP 12244-000, Brazil.
Photochem Photobiol Sci. 2023 Aug;22(8):1977-1989. doi: 10.1007/s43630-023-00426-0. Epub 2023 Apr 28.
Calcium carbonate (CaCO) exhibits a variety of crystalline phases, including the anhydrous crystalline polymorphs calcite, aragonite, and vaterite. Developing porous calcium carbonate microparticles in the vaterite phase for the encapsulation of methylene blue (MB) as a photosensitizer (PS) for use in photodynamic therapy (PDT) was the goal of this investigation. Using an adsorption approach, the PS was integrated into the CaCO microparticles. The vaterite microparticles were characterized by scanning electron microscopy (SEM) and steady-state techniques. The trypan blue exclusion method was used to measure the biological activity of macrophages infected with Leishmania braziliensis in vitro. The vaterite microparticles produced are highly porous, non-aggregated, and uniform in size. After encapsulation, the MB-loaded microparticles kept their photophysical characteristics. The carriers that were captured allowed for dye localization inside the cells. The results obtained in this study indicated that the MB-loaded vaterite microparticles show promising photodynamic activity in macrophages infected with Leishmania braziliensis.
碳酸钙（CaCO）表现出多种晶体相，包括无水结晶多晶型物方解石、文石和霰石。本研究旨在开发包封亚甲蓝（MB）作为光敏剂（PS）的水不溶性碳酸钙微孔颗粒，用于光动力疗法（PDT）。通过吸附法将 PS 整合到 CaCO 微孔颗粒中。使用扫描电子显微镜（SEM）和稳态技术对水不溶的微孔颗粒进行了表征。使用台盼蓝排除法测量体外感染利什曼原虫巴西利什曼原虫的巨噬细胞的生物活性。所制备的水不溶微孔颗粒具有高多孔性、不聚集和尺寸均匀的特点。包封后，负载 MB 的微孔颗粒保持其光物理特性。被捕获的载体允许染料在细胞内定位。本研究结果表明，负载 MB 的水不溶微孔颗粒在感染利什曼原虫巴西利什曼原虫的巨噬细胞中表现出有前景的光动力活性。
