Department of Stomatology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, PR China.
Department of Microbiology and Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, PR China.
J Med Microbiol. 2021 Mar;70(3). doi: 10.1099/jmm.0.001313. Epub 2021 Jan 18.
is an important cariogenic microbe. The potential characteristics of isolates from site-specific dental plaque are still not clear. This study aimed to investigate the phenotypic and genetic characteristics of isolates from site-specific dental plaque in China. We used isolated from children with early-childhood caries (ECC) and caries-free children to compare the phenotypic and genetic characteristics of from site-specific dental plaque samples. The ECC subjects presented two sites: a cavitated lesion and a sound surface. The caries-free subjects presented one sound surface. Growth pattern, biofilm, decrease in pH, extracellular polysaccharide, expression levels of virulence-related genes, multilocus sequence typing (MLST) and phylogenetic trees were evaluated among these three sites. The phenotypes detected between the cavitated and sound surfaces of ECC children were similar. However, the capacity for biofilm formation, pH drop and expression levels of genes ( and ) of in the caries-free group were lower compared with those of the ECC group. We identified 44 new alleles and 77 new sequence types. More than 90 % of the children with ECC shared an identical sequence type. The distribution of sequence types among different subjects showed diversity, and child-to-child transmission was detected. This is the first report of MLST on site-specific dental plaques in a single subject, and indicates that isolated from site-specific dental plaque of a single subject showed similar phenotypes as a result of the isolates were closely related.
是一种重要的致龋微生物。其特定部位牙菌斑分离株的潜在特性尚不清楚。本研究旨在探讨中国特定部位牙菌斑分离株的表型和遗传特征。我们使用从儿童早期龋（ECC）和无龋儿童中分离出的来比较特定部位牙菌斑样本中分离株的表型和遗传特征。ECC 组的研究对象有两个部位：一个是龋洞病变部位，另一个是健康表面部位。无龋组的研究对象只有一个健康表面部位。我们评估了这三个部位之间的生长模式、生物膜形成、pH 值下降、胞外多糖、毒力相关基因表达水平、多位点序列分型（MLST）和系统发育树。ECC 儿童的龋洞和健康表面之间检测到的表型相似。然而，无龋组的生物膜形成能力、pH 值下降和基因（和）表达水平均低于 ECC 组。我们鉴定了 44 个新等位基因和 77 个新序列型。超过 90%的 ECC 儿童具有相同的序列型。不同个体之间的序列型分布表现出多样性，并检测到了个体间的传播。这是首次对单个研究对象的特定部位牙菌斑进行 MLST 报道，表明来自单个研究对象特定部位牙菌斑的分离株由于分离株之间的亲缘关系密切，表现出相似的表型。
