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和 Torso 受体之间的拮抗作用调节了生殖细胞/体细胞区分的转录静止。

Antagonism between and Torso receptor regulates transcriptional quiescence underlying germline/soma distinction.

机构信息

Department of Molecular Biology, Princeton University, Princeton, United States.

Department of Cell Biology, Emory University School of Medicine, Atlanta, United States.

出版信息

Elife. 2021 Jan 18;10:e54346. doi: 10.7554/eLife.54346.

DOI:10.7554/eLife.54346
PMID:33459591
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7843132/
Abstract

Transcriptional quiescence, an evolutionarily conserved trait, distinguishes the embryonic primordial germ cells (PGCs) from their somatic neighbors. In , PGCs from embryos maternally compromised for () misexpress somatic genes, possibly resulting in PGC loss. Recent studies documented a requirement for Gcl during proteolytic degradation of the terminal patterning determinant, Torso receptor. Here we demonstrate that the somatic determinant of female fate, (), is a biologically relevant transcriptional target of Gcl. Underscoring the significance of transcriptional silencing mediated by Gcl, ectopic expression of a degradation-resistant form of Torso () can activate transcription in PGCs, whereas simultaneous loss of () reinstates the quiescent status of PGCs. Intriguingly, like mutants, embryos derived from mothers expressing in the germline display aberrant spreading of pole plasm RNAs, suggesting that mutual antagonism between Gcl and Torso ensures the controlled release of germ-plasm underlying the germline/soma distinction.

摘要

转录静止是一种进化上保守的特征，将胚胎原始生殖细胞（PGC）与其体躯邻居区分开来。在 中，母体缺陷的 （）的胚胎 PGC 错误表达体细胞基因，可能导致 PGC 丢失。最近的研究记录了 Gcl 在末端模式决定因素 Torso 受体的蛋白水解降解过程中的必需性。在这里，我们证明了雌性命运的体细胞决定因素 （）是 Gcl 的生物学相关转录靶标。强调由 Gcl 介导的转录沉默的重要性，Torso 的降解抗性形式的异位表达（）可以在 PGC 中激活 转录，而同时缺失 （）则恢复 PGC 的静止状态。有趣的是，与 突变体一样，来自表达 于生殖系中的母体的胚胎显示出极质 RNA 异常扩散，表明 Gcl 和 Torso 之间的相互拮抗作用确保了生殖系/体躯区分所基于的生殖质的受控释放。

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