Leiden J M, Gottesdiener K M, Quertermous T, Coury L, Bray R A, Gottschalk L, Gebel H, Seidman J G, Strominger J L, Landay A L
Dana-Farber Cancer Institute, Division of Tumor Virology, Boston, Massachusetts.
Immunogenetics. 1988;27(4):231-8. doi: 10.1007/BF00376117.
To test the hypothesis that the T-cell receptor (Tcr) gamma gene encodes a natural killer (NK) cell receptor molecule, three human NK clones and fresh peripheral blood lymphocytes with NK activity from two patients with a CD16+ lymphocytosis were analyzed for rearrangements and expression of the human Tcr alpha, beta, and gamma genes. Two of the clones displayed distinct rearrangements of their Tcr beta and gamma genes and expressed mature Tcr alpha, beta, and gamma RNA. However, one of the clones and both patient samples displayed marked NK activity but failed to rearrange or express any of their Tcr genes. These findings demonstrate that human natural killer activity is not dependent on Tcr gamma gene rearrangement and expression. In addition, they confirm previous findings concerning the lack of Tcr alpha and beta gene expression in some natural killer cells. Thus, they suggest the existence of additional NK-specific recognition molecules.
为了检验T细胞受体（Tcr）γ基因编码天然杀伤（NK）细胞受体分子这一假说，对来自两名CD16+淋巴细胞增多症患者的三个人类NK克隆以及具有NK活性的新鲜外周血淋巴细胞进行了分析，以检测人类Tcrα、β和γ基因的重排与表达情况。其中两个克隆显示出其Tcrβ和γ基因有明显重排，并表达成熟的Tcrα、β和γRNA。然而，其中一个克隆以及两名患者的样本均表现出显著的NK活性，但未能重排或表达其任何Tcr基因。这些发现表明，人类天然杀伤活性并不依赖于Tcrγ基因的重排与表达。此外，它们证实了先前关于某些天然杀伤细胞中缺乏Tcrα和β基因表达的发现。因此，它们提示存在其他NK特异性识别分子。
