College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, China.
Jinhua Municipal Central Hospital, Jinhua, China.
Ecotoxicol Environ Saf. 2021 Mar 15;211:111889. doi: 10.1016/j.ecoenv.2020.111889. Epub 2021 Jan 15.
The widespread use of cyclophosphamide (CP) in medical treatment had caused ubiquitous contamination in the environment. To data, many studies have been carried out on the toxic effect of CP. However, among these toxic effects of CP, which are the most sensitive remains unclear. Present study aimed to investigate the toxicity of CP on mice and evaluate the sensitivity of physiological-biochemical parameters upon exposure of mice to CP. Results showed that as compared with the control group, CP caused significant reduction in body weight (p < 0.01), spleen coefficient (p < 0.01), leukocyte density (p < 0.01) and alanine transaminase (ALT) in kidney (p < 0.01); However superoxide dismutase (SOD), malondialdehyde (MDA), ALT in liver and creatinine (Cr) in kidney significantly (p < 0.05) increased. Among the suppressed physiological and biochemical parameters, the sensitivity to CP toxicity was generally ranked as body weight > leukocyte density > ALT in kidney > spleen coefficient; while among the stimulated parameters, the sensitivity was ranked as MDA (liver) > Cr (kidney) > ALT (liver). Overall, the most sensitive parameters to CP toxicity may be associated with growth, immune system and the normal function of liver and kidney.
环磷酰胺（CP）在医学治疗中的广泛应用导致了环境中的普遍污染。迄今为止，已有许多研究致力于探讨 CP 的毒性作用。然而，CP 的这些毒性作用中，哪一种最为敏感仍不清楚。本研究旨在探讨 CP 对小鼠的毒性作用，并评估小鼠暴露于 CP 时生理生化参数的敏感性。结果表明，与对照组相比，CP 导致小鼠体重显著下降（p<0.01）、脾系数（p<0.01）、白细胞密度（p<0.01）和肾脏丙氨酸转氨酶（ALT）显著降低（p<0.01）；然而，超氧化物歧化酶（SOD）、丙二醛（MDA）、肝脏 ALT 和肾脏肌酐（Cr）显著增加（p<0.05）。在受抑制的生理生化参数中，CP 毒性的敏感性通常表现为体重>白细胞密度>肾脏 ALT>脾系数；而在受刺激的参数中，敏感性表现为 MDA（肝脏）>Cr（肾脏）>ALT（肝脏）。总的来说，对 CP 毒性最敏感的参数可能与生长、免疫系统以及肝脏和肾脏的正常功能有关。
