Elahi Narges, Astaneh Mohammad Ebrahim, Ai Jafar, Makoolati Zohreh
Students Research Committee, Fasa University of Medical Sciences, Fasa, Iran.
Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Heliyon. 2024 Aug 22;10(17):e36767. doi: 10.1016/j.heliyon.2024.e36767. eCollection 2024 Sep 15.
Cyclophosphamide (CPA) have significant effects on ovarian follicles which lead to ovarian toxicity and impair the normal female reproductive function. This study aimed to evaluate the dose-dependent effects of CPA on rat follicle numbers.
The experimental groups consisted of rats administered a single intraperitoneal injection of CPA at doses of either 50, 75,150, or 200 mg/kg followed by daily doses of 8 mg/kg for 14 days and control group given no treatment. After the treatment period, the histological evaluation was done.
Primordial and primary follicles were affected by all doses of CPA, but differential follicle counts revealed that graaf and preantral follicles were most sensitive to CPA, followed by primary and primordial follicles. The greatest reduction in all type of studied follicles caused by CPA doses of 50 mg/kg.
Differential follicle counts revealed that CPA-induced ovarian toxicity is exhibited in structural feature of the ovary, particularly in destruction of graaf and preantral follicles in a dose-dependent manner so that the highest decrease in all type of studied follicles caused by 50 mg/kg of CPA and is suggested as the best concentration for ovotoxicity induction. These findings give insight into ovarian response to structural disruption of folliculogenesis.
环磷酰胺（CPA）对卵巢卵泡有显著影响，可导致卵巢毒性并损害正常的女性生殖功能。本研究旨在评估CPA对大鼠卵泡数量的剂量依赖性影响。
实验组由单次腹腔注射CPA的大鼠组成，剂量分别为50、75、150或200mg/kg，随后每日剂量为8mg/kg，持续14天，对照组不进行处理。治疗期结束后，进行组织学评估。
所有剂量的CPA均对原始卵泡和初级卵泡有影响，但不同卵泡计数显示，格拉夫卵泡和窦前卵泡对CPA最为敏感，其次是初级卵泡和原始卵泡。50mg/kg的CPA剂量导致所有研究类型卵泡减少最多。
不同卵泡计数显示，CPA诱导的卵巢毒性表现在卵巢的结构特征上，特别是以剂量依赖性方式破坏格拉夫卵泡和窦前卵泡，因此50mg/kg的CPA导致所有研究类型卵泡减少最多，被认为是诱导卵巢毒性的最佳浓度。这些发现有助于深入了解卵巢对卵泡发生结构破坏的反应。
