Department of Chemical Engineering, École Polytechnique de Montreal, H3C 3A7, Canada.
Department of Aeronautical Engineering, Sathyabama Institute of Science and Technology, Chennai, India.
Bioresour Technol. 2021 Apr;325:124654. doi: 10.1016/j.biortech.2020.124654. Epub 2021 Jan 4.
Considering the glitches in making commercially realistic fuel, this research article has demonstrated the lipid accumulation in four fast growing, filamentous cyanobacterial strains. On day 26, the lipid content estimated was 6.7, 8.2, 10.2, and 9.4% from Phormidium sp. FW01, Phormidium sp. FW02, Oscillatoria sp. FW01, and Oscillatoria sp. FW02, respectively. Of the photosynthetically active radiation (PAR) tested, 2000 lx was found to higher biomass and lipid at about 1.83 g/L and 12.5%, respectively for Oscillatoria sp. FW01. Of <5 °C, 15 °C, 25 °C, 37-40 °C tested, 11.2% lipid was extracted from Oscillatoria sp. FW01 grown at 37-40 °C and pH did not make any changes in biomass and lipid content. The optimized abiotic conditions showed higher polar lipids about 75% in all the tested cyanobacteria and further, Oscillatoria sp. FW01 yielded 57% fatty acid methyl ester, which contains desirable fatty acids C 16:0, C 16:1, C18:1, C18:3 for high quality biodiesel.
考虑到商业上可行的燃料制造中的缺陷，本研究论文展示了四种快速生长的丝状蓝藻菌株中的脂质积累。在第 26 天，从 Phormidium sp. FW01、Phormidium sp. FW02、Oscillatoria sp. FW01 和 Oscillatoria sp. FW02 中分别估算出脂质含量为 6.7%、8.2%、10.2%和 9.4%。在测试的光合有效辐射 (PAR) 中，发现 2000 lx 更有利于生物量和脂质的产生，分别为 1.83 g/L 和 12.5%，适用于 Oscillatoria sp. FW01。在测试的 <5°C、15°C、25°C、37-40°C 中，从在 37-40°C 下生长的 Oscillatoria sp. FW01 中提取出 11.2%的脂质，并且 pH 值对生物量和脂质含量没有任何影响。优化的非生物条件显示，所有测试的蓝藻中的极性脂质含量都更高，约为 75%，此外，Oscillatoria sp. FW01 产生了 57%的脂肪酸甲酯，其中含有理想的脂肪酸 C 16:0、C 16:1、C18:1、C18:3，可用于高质量的生物柴油。
