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小球藻属和栅藻属中脂类的相对丰度,以及使用中心复合设计(CCD)改善均相酸催化条件以最大化脂肪酸甲酯产率。

Relative abundance of lipid types among Chlorella sp. and Scenedesmus sp. and ameliorating homogeneous acid catalytic conditions using central composite design (CCD) for maximizing fatty acid methyl ester yield.

机构信息

Department of Energy and Environment, National Institute of Technology, Tiruchirappalli 620015, Tamil Nadu, India.

Faculty of Aeronautical Engineering, Sathyabama Institute of Science and Technology, Chennai, India.

出版信息

Sci Total Environ. 2021 Jun 1;771:144700. doi: 10.1016/j.scitotenv.2020.144700. Epub 2021 Jan 19.

DOI:10.1016/j.scitotenv.2020.144700
PMID:33736139
Abstract

The present study has tested the biodiesel potential of two hyper lipid producing strains Chlorella sp. and Scenedesmus sp. in terms of biomass yield, quantity and quality of lipid and fatty acid composition. Biomass yield of Chlorella sp. and Scenedesmus sp. was 1.26 and 1.33 g/L, respectively on day 18 and 20. The lipid content and lipid productivity of Chlorella sp. and Scenedesmus sp. was estimated to be 21.3, 26.5% and 12.33, 14.74 mg/L/d, respectively. Notably, relative abundance of lipid types in both the strains revealed >60% neutral lipids followed by glycolipids and phospholipids in minimal level. Central composite design based optimization revealed 69 and 65.4% FAME yield from Chlorella sp. and Scenedesmus sp. at 3% sulphuric acid and 65 °C reaction temperature. Eventually, higher levels of saturated fatty acids (45%) and monounsaturated fatty acids (34%) and make Scenedesmus sp. a promising parent material for workable biodiesel production.

摘要

本研究从生物量产量、脂质数量和质量以及脂肪酸组成方面测试了两种产高油脂的藻株——小球藻和栅藻生产生物柴油的潜力。小球藻和栅藻在第 18 天和第 20 天的生物量产量分别为 1.26 和 1.33 g/L。小球藻和栅藻的脂质含量和脂质生产力估计分别为 21.3%、26.5%和 12.33、14.74 mg/L/d。值得注意的是,两种菌株的脂质类型相对丰度均显示出>60%的中性脂质,随后是低水平的糖脂和磷脂。基于中心复合设计的优化结果显示,小球藻和栅藻的 FAME 产率分别为 69%和 65.4%,硫酸用量为 3%,反应温度为 65°C。最终,较高水平的饱和脂肪酸(约 45%)和单不饱和脂肪酸(约 34%)使栅藻成为可用于生物柴油生产的有前途的母体材料。

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