Institute of Microbiology and Molecular Genetics, University of the Punjab.
Department of Microbiology and Molecular Genetics, The Women University Multan.
J Oleo Sci. 2024;73(10):1295-1310. doi: 10.5650/jos.ess23267.
Depleting fossil fuel resources and increasing energy demand have intensified the emphasis on biofuel production cyanobacteria. In this study, 19 cyanobacterial filamentous strains were isolated from various regions of Pakistan, including the northern areas and the University of the Punjab, Lahore. Sudan black, Nile red, and BODIPY staining, together with CLSM, fluorimetry, FTIR, growth in different nitrate concentrations, and GC techniques, were used to confirm and measure the lipid and diesel contents within isolated cyanobacterial filaments. Oscillatoria sp. strain CFF-6 showed a significantly higher yield (biodiesel/ biomass=35.6%). Leptolyngbya sp. strain CFF-18 showed a higher yield (biodiesel/biomass=17.1%) compared to other Leptolyngbya strains. FAME (Fatty Acid Methyl Ester) analysis was also performed. Oscillatoria are better biodiesel producers in comparison to other filamentous strains.
化石燃料资源的枯竭和能源需求的增加，使得生物燃料的生产对蓝藻的重视程度不断提高。在这项研究中，从巴基斯坦的各个地区（包括北部地区和旁遮普大学拉合尔分校）分离出了 19 株蓝藻丝状菌株。使用苏丹黑、尼罗红和 BODIPY 染色以及 CLSM、荧光计、FTIR、在不同硝酸浓度下的生长和 GC 技术，对分离的蓝藻丝状菌株中的脂质和柴油含量进行了确认和测量。Oscillatoria sp. 菌株 CFF-6 的产率（生物柴油/生物质=35.6%）明显更高。与其他 Leptolyngbya 菌株相比，Leptolyngbya sp. 菌株 CFF-18 的产率（生物柴油/生物质=17.1%）更高。还进行了 FAME（脂肪酸甲酯）分析。与其他丝状菌株相比，Oscillatoria 是更好的生物柴油生产菌。
