State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
ACS Biomater Sci Eng. 2020 May 11;6(5):2805-2811. doi: 10.1021/acsbiomaterials.0c00208. Epub 2020 Apr 3.
One simple, sensitive detection method for pesticide prothioconazole based on citrate-capped gold nanoparticles (AuNPs) in one step at room temperature was presented in this study. Prothioconazole could be easily linked onto the AuNPs through a Au-S covalent bond, and AuNPs undergo aggregation via hydrogen bonds formed among prothioconazole molecules. As a result, the color of AuNP solution changes from wine red to royal purple, which could be monitored by naked eyes and UV-vis spectra. This label-free visual detection method could determine prothioconazole with concentrations ranging from 1.33 to 19.99 μg/L with a detection limit of 0.38 μg/L (S/N = 3). This method was convenient and free of complex equipment and successfully applied to prothioconazole determination in rice field water where it gave satisfactory recoveries.
本研究提出了一种基于室温下单步合成的柠檬酸金纳米粒子（AuNPs）的简单、灵敏的农药丙硫菌唑检测方法。丙硫菌唑可以通过 Au-S 共价键轻易地连接到 AuNPs 上，并且 AuNPs 通过丙硫菌唑分子之间形成的氢键发生聚集。结果，AuNP 溶液的颜色从酒红色变为深紫色，可以通过肉眼和紫外-可见光谱进行监测。这种无标记的可视化检测方法可以检测浓度范围为 1.33 至 19.99μg/L 的丙硫菌唑，检测限为 0.38μg/L（S/N = 3）。该方法简便，无需复杂的设备，并成功应用于稻田水中丙硫菌唑的测定，回收率令人满意。
