College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang, China.
Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, The Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, Harbin, Heilongjiang, China.
Methods Mol Biol. 2023;2668:15-22. doi: 10.1007/978-1-0716-3203-1_2.
Exosomes are small extracellular vesicles that contain RNA, lipids, and proteins and can act as cellular messengers, carrying information to cells and tissues in the body. Thus, sensitive, label-free, and multiplexed analysis of exosomes may help in early diagnosis of important diseases. Here, we describe the process of pretreatment of cell-derived exosomes, preparation of surface-enhanced Raman scattering (SERS) substrates, and label-free SERS detection of exosomes using sodium borohydride aggregators. This method can enable the observation of SERS signals of exosomes that are clear and stable and have a good signal-to-noise ratio.
外泌体是一种包含 RNA、脂质和蛋白质的小型细胞外囊泡，可作为细胞信使，将信息传递到体内的细胞和组织。因此，对外泌体进行敏感、无标记和多重分析可能有助于重要疾病的早期诊断。在这里，我们描述了细胞衍生的外泌体的预处理过程、表面增强拉曼散射（SERS）基底的制备以及使用硼氢化钠聚集体的无标记 SERS 检测外泌体的方法。该方法可以实现外泌体的 SERS 信号的清晰、稳定和良好的信噪比的观察。
