Liang Haifeng, Liu Xiao, Pi Ying, Yu Qiang, Yin Yukun, Li Xian, Yang Yipei, Tian Jing
Department of Orthopedics, Zhujiang Hospital, Southern Medical University, No. 253 Industrial Avenue, Haizhu, Guangzhou 510280, People's Republic of China.
School of Materials Science and Engineering, South China University of Technology, Wushan, Guangzhou 510640, People's Republic of China.
ACS Biomater Sci Eng. 2019 Oct 14;5(10):5359-5367. doi: 10.1021/acsbiomaterials.9b00858. Epub 2019 Sep 12.
Trauma, infection, cancer, and congenital diseases can lead to bone defects. The combination of 3D printing with biomaterials is of great significance in the treatment of bone defects. In addition, pulse electromagnetic fields (PEMFs) can promote bone regeneration. The main purpose of this study was to evaluate the effects of 3D-printed scaffolds using β-tricalcium phosphate (β-TCP) as the raw material combined with a PEMF on the proliferation and differentiation of rat adipose stem cells (rADSCs) and on the repair of critical defects of the rat skull. The Cell Counting Kit-8 assay was performed to assess the proliferation of rADSCs. Alkaline phosphatase (ALP) activity, ALP staining, and the detection of osteogenic-related gene expression were performed to assess the differentiation of rADSCs. Micro-computed tomography and hematoxylin-eosin staining were used to assess the repair of rat skull defects. The results showed that the combination of the scaffold and PEMF could significantly promote the proliferation and differentiation of rADSCs and the repair of a critical defect in the rat skull. Therefore, the combination of β-TCP and PEMF with 3D printing technology can provide better treatment of clinical bone defect patients.
创伤、感染、癌症和先天性疾病均可导致骨缺损。3D打印与生物材料相结合在骨缺损治疗中具有重要意义。此外，脉冲电磁场（PEMF）可促进骨再生。本研究的主要目的是评估以β-磷酸三钙（β-TCP）为原料的3D打印支架结合PEMF对大鼠脂肪干细胞（rADSCs）增殖和分化以及对大鼠颅骨关键缺损修复的影响。采用细胞计数试剂盒-8法评估rADSCs的增殖。进行碱性磷酸酶（ALP）活性、ALP染色及成骨相关基因表达检测以评估rADSCs的分化。采用微型计算机断层扫描和苏木精-伊红染色评估大鼠颅骨缺损的修复情况。结果表明，支架与PEMF联合应用可显著促进rADSCs的增殖和分化以及大鼠颅骨关键缺损的修复。因此，β-TCP和PEMF与3D打印技术相结合可为临床骨缺损患者提供更好的治疗。
