长链非编码 RNA NEAT1 上 SNP 的变异与中国汉族人群的胃癌易感性有关。
Variant of SNPs at lncRNA NEAT1 contributes to gastric cancer susceptibility in Chinese Han population.
机构信息
Department of Epidemiology, College of Public Health, Zhengzhou University, No 100 Kexue Avenue, Zhengzhou City, 450001, China.
Key Laboratory of Tumor Epidemiology of Henan Province, Zhengzhou University, Zhengzhou, China.
出版信息
Int J Clin Oncol. 2021 Apr;26(4):694-700. doi: 10.1007/s10147-020-01852-1. Epub 2021 Jan 19.
BACKGROUND
The long noncoding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) has been implicated in many tumors risk including gastric cancer. However, the association of single nucleotide polymorphisms (SNPs) at NEAT1 with gastric cancer risk has not been studied. The aim of this study was to investigate the association between SNPs in NEAT1 and gastric cancer susceptibility.
METHODS
In this study, four SNPs in lncRNA NEAT1 were selected for genotyping in 484 gastric cancer patients and 484 controls in Chinese Han population. Quantitative real-time PCR (qRT-PCR) was conducted to evaluate the potential function of rs3825071. Attributable risk percentage (ARP) and population attributable risk percentage (PARP) were used to assess the epidemiological effect.
RESULTS
In the dominant model (GG), the genotypes AG + AA of rs3825071 and rs7943779 were associated with an increased risk of gastric cancer (OR = 1.72, 95%CI = 1.27-2.32 and OR = 1.63, 95%CI = 1.19-2.22). Individuals harboring ≥ 3 risk alleles have higher risk of gastric cancer (OR = 1.88, 95% CI = 1.26-2.80, P = 0.002). ARP and PARP associated with gastric cancer were 42.53% and 10.88% for rs3825071, and were 33.78% and 6.26% for rs7943779, respectively. Furthermore, compared with the genotype GG of rs3825071, the genotypes AG and AA had higher expression of NEAT1.
CONCLUSIONS
We found that the genetic variations in NEAT1 were significantly associated with risk of gastric cancer. The G > A variant of rs3825071 may confer gastric cancer susceptibility by changed biological effects to increase the expression of NEAT1.
背景
长链非编码 RNA（lncRNA）核富集丰富转录本 1（NEAT1）已被涉及到许多肿瘤风险，包括胃癌。然而，NEAT1 上的单核苷酸多态性（SNP）与胃癌风险的关联尚未被研究。本研究旨在探讨 NEAT1 中的 SNP 与胃癌易感性之间的关系。
方法
在这项研究中，选择了 lncRNA NEAT1 中的四个 SNP 在中国汉族人群中进行 484 例胃癌患者和 484 例对照的基因分型。定量实时 PCR（qRT-PCR）用于评估 rs3825071 的潜在功能。归因风险百分比（ARP）和人群归因风险百分比（PARP）用于评估流行病学效应。
结果
在显性模型（GG）中，rs3825071 和 rs7943779 的 AG+AA 基因型与胃癌风险增加相关（OR=1.72，95%CI=1.27-2.32 和 OR=1.63，95%CI=1.19-2.22）。携带≥3 个风险等位基因的个体患胃癌的风险更高（OR=1.88，95%CI=1.26-2.80，P=0.002）。与胃癌相关的 ARP 和 PARP 分别为 rs3825071 的 42.53%和 10.88%，rs7943779 的 33.78%和 6.26%。此外，与 rs3825071 的 GG 基因型相比，AG 和 AA 基因型的 NEAT1 表达更高。
结论
我们发现 NEAT1 的遗传变异与胃癌风险显著相关。rs3825071 的 G>A 变体可能通过改变生物学效应来增加 NEAT1 的表达，从而导致胃癌易感性。
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