CKMT1B 是低级别神经胶质瘤的一个潜在预后生物标志物，并与免疫浸润相关。
CKMT1B is a potential prognostic biomarker and associated with immune infiltration in Lower-grade glioma.
机构信息
Department of Internal Medicine, Zhanjiang Central Hospital, Guangdong Medical University, Zhanjiang, Guangdong Province, China.
Department of Oncology, Guangdong Medical University, Zhanjiang, Guangdong Province, China.
出版信息
PLoS One. 2021 Jan 19;16(1):e0245524. doi: 10.1371/journal.pone.0245524. eCollection 2021.
BACKGROUND
Lower-grade glioma (LGG) is the most common histology identified in glioma. CKMT1B has not been investigated in glioma. The purpose of this research was to investigate the prognostic value of CKMT1B and its correlation with immune infiltration in LGG.
METHODS
We used Gene Expression Profiling Interactive Analysis (GEPIA) to analyze the expression of CKMT1B in LGG. Univariate and multivariate Cox regression analyses were used to assess the effect of CKMT1B expression and screened variables on survival. The correlation between CKMT1B and immune infiltration was evaluated by TIMER and CIBERSORT. Moreover, the possible biological functions of CKMT1B were studied by GSEA. The statistical analysis was conducted by R software.
RESULTS
The expression of CKMT1B was significantly lower than the normal samples in LGG. Low expression of CKMT1B predicts a worse prognosis. Multivariate Cox analyses revealed that CKMT1B might be an independent favorable prognostic indicator. TIMER analysis revealed that CKMT1B expression level was related to immune infiltration. CIBERSORT analysis showed that CKMT1B expression was positively related to the infiltration level of activated mast cells and negatively related to macrophage M2 in LGG. Moreover, GESA showed that multiple cancer-related and immune-related gene sets were enriched in the low-CKMT1B group in the top 5 of the most significant differences.
CONCLUSION
CKMT1B is a prognostic biomarker with potential applications and associated with immune infiltration in Lower-grade glioma.
背景
低级别胶质瘤（LGG）是胶质瘤中最常见的组织学类型。CKMT1B 在胶质瘤中尚未得到研究。本研究旨在探讨 CKMT1B 在 LGG 中的预后价值及其与免疫浸润的相关性。
方法
我们使用基因表达谱交互分析（GEPIA）分析 CKMT1B 在 LGG 中的表达。采用单因素和多因素 Cox 回归分析评估 CKMT1B 表达和筛选变量对生存的影响。通过 TIMER 和 CIBERSORT 评估 CKMT1B 与免疫浸润的相关性。此外，通过 GSEA 研究 CKMT1B 的可能生物学功能。统计分析采用 R 软件进行。
结果
CKMT1B 在 LGG 中的表达明显低于正常样本。CKMT1B 低表达预示预后不良。多因素 Cox 分析表明，CKMT1B 可能是一个独立的有利预后指标。TIMER 分析表明，CKMT1B 表达水平与免疫浸润有关。CIBERSORT 分析显示，CKMT1B 表达与 LGG 中激活肥大细胞的浸润水平呈正相关，与巨噬细胞 M2 呈负相关。此外，GSEA 显示，在差异最显著的前 5 位中，低 CKMT1B 组富集了多个与癌症相关和免疫相关的基因集。
结论
CKMT1B 是一种具有潜在应用价值的预后生物标志物，与 LGG 中的免疫浸润有关。
Zotero 插件