Shirani Maryam, Kalantari Heibatullah, Khodayar Mohammad Javad, Kouchak Maryam, Rahbar Nadereh
Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Pharmaceutics, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Apr 5;250:119345. doi: 10.1016/j.saa.2020.119345. Epub 2020 Dec 22.
A novel nanobiosensor was prepared by aptamer and gold nanoparticles conjugate in poly vinyl alcohol hydrogel for sensitive detection of digoxin in human plasma samples. The developed nanobiosensor was characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and dynamic light scattering instrument. In this sensor the hydrogel acted as a fluorescent probe. The fluorescence intensity of the hydrogel was quenched by aptamer stabilized gold nanoparticles as energy acceptor. Upon addition of digoxin, the aptamer/drug complex was formed and the fluorescence of the hydrogel was restored because of destabilization and aggregation of gold nanoparticles in the presence of salt. The affecting parameters on the nanobiosensor performance were assessed and under the optimized conditions the external and in plasma calibration curves were linear in the 10-1000 ng L digoxin concentration range with detection limits of 2.9 and 3.1 ng L, respectively. The relative standard deviations for 5 replicate determinations of 50, 250, and 500 ng L of digoxin, were 7.3, 5.1, and 3.8%, respectively. This nanofluoroprobe was successfully applied for determination of digoxin in spiked plasma samples without any pretreatment procedure.
通过将适配体与金纳米颗粒共轭于聚乙烯醇水凝胶中制备了一种新型纳米生物传感器，用于灵敏检测人血浆样本中的地高辛。所开发的纳米生物传感器通过傅里叶变换红外光谱、透射电子显微镜和动态光散射仪进行了表征。在该传感器中，水凝胶充当荧光探针。水凝胶的荧光强度被作为能量受体的适配体稳定化金纳米颗粒淬灭。加入地高辛后，形成了适配体/药物复合物，并且由于盐存在下金纳米颗粒的去稳定化和聚集，水凝胶的荧光得以恢复。评估了影响纳米生物传感器性能的参数，在优化条件下，外部校准曲线和血浆校准曲线在10 - 1000 ng/L地高辛浓度范围内呈线性，检测限分别为2.9和3.1 ng/L。对50、250和500 ng/L地高辛进行5次重复测定的相对标准偏差分别为7.3%、5.1%和3.8%。这种纳米荧光探针无需任何预处理程序就成功应用于加标血浆样本中地高辛的测定。
