Fernandes João S, Martins Margarida, Neves Nuno M, Fernandes Maria H V, Reis Rui L, Pires Ricardo A
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark-Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco GMR, Portugal.
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, 4710-057 Portugal.
ACS Biomater Sci Eng. 2016 Jul 11;2(7):1143-1150. doi: 10.1021/acsbiomaterials.6b00162. Epub 2016 Jun 29.
Three novel borosilicate bioactive glasses (BBGs) were prepared and used to investigate their bioactive and antibacterial properties. The BBGs were prepared by melt-quenching using different glass modifiers, i.e. Mg, Ca, and Sr, and their amorphous nature was confirmed by X-ray diffraction. Scanning electron microscopy with energy dispersive X-ray spectroscopy allowed the visualization of apatite-like structures upon 7 days of immersion in simulated body fluid. BBG-Ca generated surface structures with a Ca/P ratio ≈1.67, while the surface of the BBG-Sr was populated with structures with a Sr/P ratio ≈1.7. Moreover, bacterial tests showed that the BBG-Mg and BBG-Sr glasses (at concentrations of 9, 18, 36, and 72 mg/mL) present antibacterial characteristics. In particular, BBG-Sr, at concentrations of 9 mg/mL, exhibited bacteriostatic activity against , and at concentrations ≥18 mg/mL it was able to eradicate this bacterium. These results evidence an antibacterial activity dependent on the BBGs composition, concentration, and bacterial species. Cellular studies showed that the developed BBGs do not present a statistically significant cytotoxic effect against Saos-2 cells after 3 days of culture, showing better performance (in the cases of BBG-Ca and BBG-Sr) than commercial 45S5 Bioglass up to 7 days of culture. Overall, this study demonstrates that BBGs can be effectively designed to combine bioactivity and intrinsic antibacterial activity targeting bone tissue engineering applications.
制备了三种新型硼硅酸盐生物活性玻璃（BBG），并对其生物活性和抗菌性能进行了研究。通过使用不同的玻璃改性剂（即Mg、Ca和Sr）进行熔融淬火制备了BBG，并通过X射线衍射确认了它们的非晶态性质。扫描电子显微镜结合能量色散X射线光谱法可以观察到在模拟体液中浸泡7天后的类磷灰石结构。BBG-Ca生成了Ca/P比约为1.67的表面结构，而BBG-Sr的表面则布满了Sr/P比约为1.7的结构。此外，细菌测试表明，BBG-Mg和BBG-Sr玻璃（浓度分别为9、18、36和72 mg/mL）具有抗菌特性。特别是，浓度为9 mg/mL的BBG-Sr对[细菌名称未给出]表现出抑菌活性，浓度≥18 mg/mL时能够根除这种细菌。这些结果证明了抗菌活性取决于BBG的组成、浓度和细菌种类。细胞研究表明，所开发的BBG在培养3天后对Saos-2细胞没有统计学上显著的细胞毒性作用，在培养7天内（在BBG-Ca和BBG-Sr的情况下）表现出比商业45S5生物玻璃更好的性能。总体而言，这项研究表明，可以有效地设计BBG，以结合针对骨组织工程应用的生物活性和内在抗菌活性。
