Abodunrin Oluwatosin David, El Mabrouk Khalil, Bricha Meriame
Euromed University of Fes, UEMF, Morocco.
J Mater Chem B. 2025 Feb 5;13(6):2100-2113. doi: 10.1039/d4tb02416h.
Bone defects resulting from trauma or diseases that lead to bone loss have created a growing need for innovative materials suitable for treating bone-related conditions. The purpose of this study is, therefore, to synthesize and analyse the synergistic effects of cerium (Ce) and cerium-silver (Ce-Ag) doping of borosilicate bioactive glass (BBG) on the bioactivity, antibacterial properties, and biocompatibility for potential applications in bone tissue engineering. This study utilized a sol-gel Stöber method to synthesize doped BBGs based on S49B4. Characterization techniques were utilized to evaluate the thermal stability, elemental composition, structural integrity, and morphological properties of the synthesized Ce and AgCe-BBGs. Cytotoxicity was evaluated using a GMSM-K gingival cell line, while antimicrobial tests were conducted using clinical isolates of and . The characterization results confirmed the successful incorporation of Ce and Ag, resulting in elongated pineal to spherical nanosized BG particles (33-68 nm). Thermal analysis indicated that silver exhibited lower thermal stability compared to cerium. Bioactivity tests indicated that while silver has intrinsic bioactive qualities, elevated cerium levels above 0.5 wt% may inhibit or delay apatite formation by generating insoluble cerium phosphate ions. Lactate dehydrogenase assays demonstrated that among other BBGs, SBAgCe1 showed the highest LDH activity, suggesting mild cytotoxicity. The co-doped BBG exhibited strong antibacterial activity through a complex interaction between Ag and Ce ionic exchange. Nonetheless, a careful balance of Ce and Ag concentrations is critical, as high levels can compromise bioactivity and increase cytotoxicity. The results highlight the potential of SBAgCe0.5 as a candidate for bone tissue engineering applications due to its favourable bioactivity, and antibacterial and cytocompatible properties, emphasizing the importance of optimizing dopant concentrations for therapeutic applications in favour of good health and the well-being of humanity.
由创伤或导致骨质流失的疾病引起的骨缺损，使得对适用于治疗骨相关病症的创新材料的需求日益增长。因此，本研究的目的是合成并分析硼硅酸盐生物活性玻璃（BBG）的铈（Ce）和铈银（Ce-Ag）掺杂对生物活性、抗菌性能和生物相容性的协同效应，以用于骨组织工程的潜在应用。本研究利用溶胶-凝胶Stöber方法基于S49B4合成掺杂的BBG。采用表征技术评估合成的Ce-BBG和AgCe-BBG的热稳定性、元素组成、结构完整性和形态学性质。使用GMSM-K牙龈细胞系评估细胞毒性，同时使用 和 的临床分离株进行抗菌测试。表征结果证实成功掺入了Ce和Ag，形成了从细长松果状到球形的纳米级BG颗粒（33-68nm）。热分析表明，银的热稳定性低于铈。生物活性测试表明，虽然银具有内在的生物活性特性，但铈含量高于0.5wt%时，可能会通过生成不溶性磷酸铈离子来抑制或延迟磷灰石形成。乳酸脱氢酶测定表明，在其他BBG中，SBAgCe1表现出最高的LDH活性，表明具有轻度细胞毒性。通过Ag和Ce离子交换之间的复杂相互作用，共掺杂的BBG表现出很强抗菌活性。然而，Ce和Ag浓度的仔细平衡至关重要，因为高浓度会损害生物活性并增加细胞毒性。结果突出了SBAgCe0.5作为骨组织工程应用候选材料的潜力，因其具有良好的生物活性、抗菌和细胞相容性，强调了优化掺杂剂浓度对于有利于人类健康和福祉的治疗应用的重要性。
