Hofmann Marika, Heine Thomas, Malik Luise, Hofmann Sarah, Joffroy Kristin, Senges Christoph Helmut Rudi, Bandow Julia Elisabeth, Tischler Dirk
Institute of Biosciences, TU Bergakademie Freiberg, 09599 Freiberg, Germany.
Applied Microbiology, Faculty of Biology and Biotechnology, Ruhr-Universität Bochum, 44780 Bochum, Germany.
Microorganisms. 2021 Jan 5;9(1):111. doi: 10.3390/microorganisms9010111.
To guarantee the supply of critical elements in the future, the development of new technologies is essential. Siderophores have high potential in the recovery and recycling of valuable metals due to their metal-chelating properties. Using the Chrome azurol S assay, 75 bacterial strains were screened to obtain a high-yield siderophore with the ability to complex valuable critical metal ions. The siderophore production of the four selected strains 3E, DSM 50083, EPS, and B7g was optimized, resulting in significantly increased siderophore production of and . Produced siderophore amounts and velocities were highly dependent on the carbon source. The genomes of and were sequenced. Bioinformatical analyses revealed the occurrence of an achromobactin and a pyoverdine gene cluster in , a heterobactin and a requichelin gene cluster in , and a desferrioxamine gene cluster in Finally, the results of the previous metal-binding screening were validated by a proof-of-concept development for the recovery of metal ions from aqueous solutions utilizing C columns functionalized with siderophores. We demonstrated the recovery of the critical metal ions V(III), Ga(III), and In(III) from mixed metal solutions with immobilized siderophores of and
为保证未来关键元素的供应，新技术的开发至关重要。由于其金属螯合特性，铁载体在回收和循环利用有价值金属方面具有巨大潜力。使用铬天青S测定法，筛选了75株细菌菌株，以获得一种能够络合有价值的关键金属离子的高产铁载体。对所选的四株菌株3E、DSM 50083、EPS和B7g的铁载体产量进行了优化，结果铁载体产量显著提高。所产生的铁载体数量和速度高度依赖于碳源。对3E和B7g的基因组进行了测序。生物信息学分析表明，3E中存在无色杆菌素和绿脓菌素基因簇，EPS中存在杂菌素和requichelin基因簇，B7g中存在去铁胺基因簇。最后，通过利用用铁载体功能化的C柱从水溶液中回收金属离子的概念验证开发，验证了先前金属结合筛选的结果。我们展示了用3E和B7g的固定化铁载体从混合金属溶液中回收关键金属离子V(III)、Ga(III)和In(III)的过程。
