Koedam N, Wittouck E, Gaballa A, Gillis A, Höfte M, Cornelis P
Unit of Plant Physiology, Vrije Universiteit Brussel, Belgium.
Biometals. 1994 Oct;7(4):287-91. doi: 10.1007/BF00144123.
Siderophores are microbial, low molecular weight iron-chelating compounds. Fluorescent Pseudomonads produce different, strain-specific fluorescent siderophores (pyoverdines) as well as non-fluorescent siderophores in response to low iron conditions. We present an isoelectric focusing method applicable to unpurified as well as to purified pyoverdine samples where the fluorescent siderophores are visualized under UV illumination. Siderophores from different Pseudomonas sp., amongst which are P. aeruginosa, P. fluorescens and P. putida, including egg yolk, rhizospheric and clinical isolates as well as some derived Tn5 mutants were separated by this technique. Different patterns could be observed for strains known to produce different siderophores. The application of the chrome azurol S assay as a gel overlay further allows immediate detection of non-fluorescent siderophores or possibly degradation products with residual siderophore activity. The method was also applied to other microbial siderophores such as deferrioxamine B.
铁载体是微生物产生的低分子量铁螯合化合物。荧光假单胞菌在低铁条件下会产生不同的、菌株特异性的荧光铁载体（绿脓菌素）以及非荧光铁载体。我们提出了一种等电聚焦方法，该方法适用于未纯化的以及纯化的绿脓菌素样品，在紫外光照射下可以观察到荧光铁载体。通过该技术分离了来自不同假单胞菌属的铁载体，其中包括铜绿假单胞菌、荧光假单胞菌和恶臭假单胞菌，包括蛋黄、根际和临床分离株以及一些衍生的Tn5突变体。对于已知产生不同铁载体的菌株，可以观察到不同的模式。将铬天青S测定法作为凝胶覆盖物应用，进一步可以立即检测非荧光铁载体或可能具有残留铁载体活性的降解产物。该方法也应用于其他微生物铁载体，如去铁胺B。
