Nasso Rosarita, Pagliara Valentina, D'Angelo Stefania, Rullo Rosario, Masullo Mariorosario, Arcone Rosaria
Dipartimento di Scienze Motorie e del Benessere, Università degli Studi di Napoli "Parthenope", Via Medina 40, 80133 Napoli, Italy.
ISPAAM, Consiglio Nazionale delle Ricerche, Piazzale Enrico Fermi 1, 80055 Portici, Italy.
Pharmaceuticals (Basel). 2021 Jan 14;14(1):62. doi: 10.3390/ph14010062.
In this study, we explored the ability of apple flesh polyphenol extract (AFPE) to affect the activity of key enzymes involved in neurodegenerative disorders-in particular, Acetyl- and Butirryl-cholinesterases, and type A and B monoamine oxidase. The effect of AFPE on enzyme activity was analyzed by in vitro enzyme assays, and the results showed concentration-dependent enzyme inhibition, with IC values corresponding to 859 ± 18 µM and 966 ± 72 µM for AChE and BuChE respectively, and IC corresponding to 145 ± 3 µM and 199 ± 7 µM for MAO-A and MAO-B, respectively, with a preference for MAO-A. Moreover, in this concentration range, AFPE did not affect the viability of human neuroblastoma SH-SY5Y and fibroblast BJ-5ta cell lines, as determined by an MTT assay. In conclusion, our results demonstrate that AFPE shows the new biological properties of inhibiting the activity of enzymes that are involved in brain functions, neurodegenerative disorders, and aging.
在本研究中，我们探究了苹果果肉多酚提取物（AFPE）影响神经退行性疾病相关关键酶活性的能力，特别是乙酰胆碱酯酶和丁酰胆碱酯酶，以及A型和B型单胺氧化酶。通过体外酶分析测定AFPE对酶活性的影响，结果显示出浓度依赖性的酶抑制作用，乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）的半数抑制浓度（IC）值分别为859±18 μM和966±72 μM，单胺氧化酶A型（MAO-A）和单胺氧化酶B型（MAO-B）的IC值分别为145±3 μM和199±7 μM，且对MAO-A的抑制作用更明显。此外，在该浓度范围内，通过MTT法测定发现AFPE不影响人神经母细胞瘤SH-SY5Y细胞系和成纤维细胞BJ-5ta细胞系的活力。总之，我们的结果表明，AFPE具有抑制参与脑功能、神经退行性疾病和衰老的酶活性的新生物学特性。
