Hayat Mohammad, Mohd Yusoff Md Sabri, Samad Mohd Jamil, Abdul Razak Intan Shameha, Md Yasin Ina Salwany, Thompson Kim D, Hasni Khalil
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia.
Aquatic Animal Health and Therapeutics Laboratory, Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia.
Vaccines (Basel). 2021 Jan 14;9(1):51. doi: 10.3390/vaccines9010051.
Red hybrid tilapia were fed a formalin-killed oral vaccine (FKV) in the present study was assessed. Three hundred Red hybrid tilapia 80 ± 10 g were divided into five groups (1A, 1B, 2A, 2B, and Cx), each consisting of 60 fish. Fish from Groups 1A, 1B, 2A, and 2B were fed with FKV over different periods of administration, while Group 2B was the only group of fish to receive an oral booster vaccination on day 14- and 21-days post-vaccination (dpv). Group Cx was fed with normal pellets containing no vaccine as a control group. At four weeks post-vaccination (wpv), all fish were experimentally infected with . Groups 2A and 2B had the lowest level of mortalities following vaccination (45% and 30%, respectively) compared to Groups 1A and 1B (80% and 55%, respectively), while the level of mortalities in Group Cx was 100%. All vaccinated groups showed a significant increase in anti- IgM levels ( < 0.05) in serum, mucus, and gut-lavage, while Group Cx did not ( > 0.05) and all fish in this group died by five weeks post-infection. In conclusion, fish fed with the FKV had a greater level of protection against , with increased specific antibody response to the vaccine and there was also evidence of GALT stimulation by the vaccine.
在本研究中，对红色杂交罗非鱼投喂福尔马林灭活口服疫苗（FKV）进行了评估。将300条体重80±10克的红色杂交罗非鱼分为五组（1A、1B、2A、2B和Cx），每组60条鱼。1A、1B、2A和2B组的鱼在不同的给药期投喂FKV，而2B组是唯一在接种疫苗后第14天和第21天接受口服加强免疫的鱼组。Cx组投喂不含疫苗的正常颗粒饲料作为对照组。在接种疫苗后四周（wpv），所有鱼都进行了实验性感染。与1A组和1B组（分别为80%和55%）相比，2A组和2B组在接种疫苗后的死亡率最低（分别为45%和30%），而Cx组的死亡率为100%。所有接种疫苗的组血清、黏液和肠道灌洗液中的抗IgM水平均显著升高（P<0.05），而Cx组则没有（P>0.05），该组所有鱼在感染后五周内死亡。总之，投喂FKV的鱼对[病原体未明确]具有更高的保护水平，对疫苗的特异性抗体反应增强，并且也有证据表明疫苗刺激了肠道相关淋巴组织（GALT）。
