Department of Surgery, Medicine, Dentistry and Morphological Sciences with Interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, 41125 Modena, Italy.
Operative Unit of Dentistry and Maxillofacial Surgery, Department Integrated Activity-Specialist Surgeries, University-Hospital of Modena, 41125 Modena, Italy.
Int J Mol Sci. 2021 Jan 16;22(2):865. doi: 10.3390/ijms22020865.
Dental implants are one of the most frequently used treatment options for tooth replacement, and titanium is the metal of choice due to its demonstrated superiority in resisting corrosion, lack of allergic reactions and mechanical strength. Surface roughness of titanium implants favors the osseointegration process; nevertheless, its topography may provide a suitable substrate for bacterial biofilm deposition, causing peri-implantitis and leading to implant failure. Subgingival prophylaxis treatments with cleansing powders aimed to remove the bacterial accumulation are under investigation. Two different air-polishing powders-glycine and tagatose-were assayed for their cleaning and antimicrobial potential against a biofilm and for their effects on human dental pulp stem cells (hDPSCs), seeded on sandblasted titanium disks. Immunofluorescence analyses were carried out to evaluate cell adhesion, proliferation, stemness and osteogenic differentiation. The results demonstrate that both the powders have a great in vitro cleaning potential in the early period and do not show any negative effects during hDPSCs osteogenic differentiation process, suggesting their suitability for enhancing the biocompatibility of titanium implants. Our data suggest that the evaluated cleansing systems reduce microbial contamination and allow us to propose tagatose as an adequate alternative to the gold standard glycine for the air-polishing prophylaxis treatment.
种植牙是目前应用最广泛的一种牙齿修复方式，而钛因其在抗腐蚀、无过敏反应和机械强度方面的优势，成为首选金属。钛种植体的表面粗糙度有利于骨整合过程；然而，其表面形貌可能为细菌生物膜的沉积提供合适的基质，导致种植体周围炎，最终导致种植体失败。目前正在研究使用清洁粉末进行龈下预防治疗，以清除细菌堆积。我们对两种不同的空气抛光粉末——甘氨酸和塔格糖——进行了测试，以评估它们对生物膜的清洁和抗菌潜力，以及对种植在喷砂钛盘上的人牙髓干细胞（hDPSCs）的影响。通过免疫荧光分析来评估细胞黏附、增殖、干性和成骨分化。结果表明，这两种粉末在早期都具有很强的体外清洁能力，而且在 hDPSCs 成骨分化过程中没有任何负面作用，这表明它们适合提高钛种植体的生物相容性。我们的数据表明，所评估的清洁系统减少了微生物污染，并使我们能够提出塔格糖作为空气抛光预防治疗中金标准甘氨酸的合适替代品。
