Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.
Department of Polymer Science and Engineering, Kyungpook National University, Daegu 41566, Korea.
Int J Mol Sci. 2019 Aug 5;20(15):3812. doi: 10.3390/ijms20153812.
The cellular distribution of silica nanoparticles (NPs) in the liver is not well understood. Targeting specific cells is one of the most important issues in NP-based drug delivery to improve delivery efficacy. In this context, the present study analyzed the relative cellular distribution pattern of silica NPs in the liver, and the effect of surface energy modification on NPs. Hydrophobic NP surface modification enhanced NP delivery to the liver and liver sinusoid fFendothelial cells (LSECs). Conversely, hydrophilic NP surface modification was commensurate with targeting hepatic stellate cells (HSCs) rather than other cell types. There was no notable difference in NP delivery to Kupffer cells or hepatocytes, regardless of hydrophilic or hydrophobic NP surface modification, suggesting that both the targeting of hepatocytes and evasion of phagocytosis by Kupffer cells are not associated with surface energy modification of silica NPs. This study provides useful information to target specific cell types using silica NPs, as well as to understand the relationship between NP surface energy and the NP distribution pattern in the liver, thereby helping to establish strategies for cell targeting using various NPs.
硅纳米颗粒（NPs）在肝脏中的细胞分布尚不清楚。靶向特定细胞是基于 NP 的药物输送以提高输送效果的最重要问题之一。在这种情况下，本研究分析了肝脏中硅 NPs 的相对细胞分布模式，以及表面能修饰对 NPs 的影响。疏水性 NP 表面修饰增强了 NP 向肝脏和肝窦内皮细胞（LSECs）的输送。相反，亲水性 NP 表面修饰与靶向肝星状细胞（HSCs）而不是其他细胞类型相称。亲水性或疏水性 NP 表面修饰对库普弗细胞或肝细胞中 NP 的输送均无明显差异，这表明 NP 靶向肝细胞和逃避库普弗细胞吞噬作用均与硅 NP 的表面能修饰无关。本研究为使用硅 NPs 靶向特定细胞类型提供了有用的信息，并有助于理解 NP 表面能与 NP 在肝脏中的分布模式之间的关系，从而有助于建立使用各种 NPs 进行细胞靶向的策略。
