通过蓝藻喂食进行的RNA干扰表明该基因在[具体生物名称]口腔器官再生过程中的纤毛发生中至关重要。

RNA Interference by Cyanobacterial Feeding Demonstrates the Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in .

作者信息

Wei Wei, Jiang Chuanqi, Chai Xiaocui, Zhang Juyuan, Zhang Cheng-Cai, Miao Wei, Xiong Jie

机构信息

Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China.

出版信息

Microorganisms. 2021 Jan 15;9(1):176. doi: 10.3390/microorganisms9010176.

DOI:10.3390/microorganisms9010176

PMID:33467569

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7830263/

Abstract

In the giant ciliate , oral apparatus (OA) regeneration is an experimentally tractable regeneration paradigm that occurs via a series of morphological steps. OA regeneration is thought to be driven by a complex regulatory system that orchestrates the temporal expression of conserved and specific genes. We previously identified a -specific gene (named ) that was significantly upregulated during the ciliogenesis stages of OA regeneration, with an expression peak at the stage of the first OA cilia appearance. We established a novel RNA interference (RNAi) method through cyanobacteria sp. PCC6803 feeding in . The expression of gene was significantly knocked down by using this method and induced abnormal ciliogenesis of OA regeneration in , suggesting that is essential for OA regeneration in . This novel RNAi method by cyanobacterial feeding has potential utility for studying other ciliates.

摘要

在大型纤毛虫中，口器（OA）再生是一种可通过一系列形态学步骤进行实验研究的再生模式。OA再生被认为是由一个复杂的调控系统驱动的，该系统协调保守和特定基因的时间表达。我们之前鉴定出一个特定基因（命名为），它在OA再生的纤毛发生阶段显著上调，在第一个OA纤毛出现阶段表达达到峰值。我们通过在中投喂蓝藻PCC6803建立了一种新的RNA干扰（RNAi）方法。使用该方法基因的表达被显著敲低，并在中诱导了OA再生的异常纤毛发生，这表明对中的OA再生至关重要。这种通过蓝藻投喂的新型RNAi方法在研究其他纤毛虫方面具有潜在用途。