Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Gene. 2020 Jun 15;743:144624. doi: 10.1016/j.gene.2020.144624. Epub 2020 Mar 26.
The giant ciliate Stentor coeruleus (S. coeruleus) is a suitable model organism for studying morphogenesis and regeneration at the single-cell level. It contains a prominent structure on the anterior end of the cell, known as the oral apparatus (OA). OA can be induced to shed by urea treatment and then new OA regenerates via a series of defined morphological events and the cell resumes normal feeding activity. We identified OA constituents in S. coeruleus by mass spectrometry. A total of 882 OA-associated proteins were identified; the homologs of 181 of these are known OA constituents in other organisms. The expression pattern of OA-associated genes during regeneration was investigated using single-cell transcriptome sequencing. The expression of most OA-associated genes was high during regeneration, indicating their stable expression after OA shedding. We also identified OA-associated differentially expressed genes that may be involved in regulating OA reconstruction. In summary, this study gives preliminary insight into the molecular basis of OA in S. coeruleus.
巨大草履虫（Stentor coeruleus）是一种适用于在单细胞水平上研究形态发生和再生的模式生物。它的细胞前端有一个明显的结构，称为口器（OA）。通过尿素处理可以诱导 OA 脱落，然后通过一系列明确的形态发生事件，新的 OA 会再生，细胞恢复正常的摄食活动。我们通过质谱法鉴定了 S. coeruleus 中的 OA 组成成分。总共鉴定出 882 种与 OA 相关的蛋白质；其中 181 种的同源物是其他生物体中已知的 OA 组成成分。使用单细胞转录组测序研究了再生过程中与 OA 相关的基因的表达模式。大多数与 OA 相关的基因在再生过程中表达量很高，这表明它们在 OA 脱落之后稳定表达。我们还鉴定了与 OA 相关的差异表达基因，这些基因可能参与调节 OA 的重建。总之，本研究初步揭示了 S. coeruleus 中 OA 的分子基础。
