Rahmani A, Jinap S, Soleimany F
Authors are with Center of Excellence for Food Safety Research (CEFSR), Faculty of Food Science and Technology, Univ. Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. Direct inquiries to author Jinap (E-mail:
Compr Rev Food Sci Food Saf. 2009 Jul;8(3):202-251. doi: 10.1111/j.1541-4337.2009.00079.x.
Mycotoxin toxicity occurs at very low concentrations, therefore sensitive and reliable methods for their detection are required. Consequently, sampling and analysis of mycotoxins is of critical importance because failure to achieve a suitable verified analysis can lead to unacceptable consignments being accepted or satisfactory shipments unnecessarily rejected. The general mycotoxin analyses carried out in laboratories are still based on physicochemical methods, which are continually improved. Further research in mycotoxin analysis has been established in such techniques as screening methods with TLC, GC, HPLC, and LC-MS. In some areas of mycotoxin method development, immunoaffinity columns and multifunctional columns are good choices as cleanup methods. They are appropriate to displace conventional liquid-liquid partitioning or column chromatography cleanup. On the other hand, the need for rapid yes/no decisions for exported or imported products has led to a number of new screening methods, mainly, rapid and easy-to-use test kits based on immuno-analytical principles. In view of the fact that analytical methods for detecting mycotoxins have become more prevalent, sensitive, and specific, surveillance of foods for mycotoxin contamination has become more commonplace. Reliability of methods and well-defined performance characteristics are essential for method validation. This article covers some of the latest activities and progress in qualitative and quantitative mycotoxin analysis.
霉菌毒素在极低浓度下就会产生毒性，因此需要灵敏可靠的检测方法。所以，霉菌毒素的采样和分析至关重要，因为如果未能进行合适的验证分析，可能会导致不合格货物被接收，或者合格货物被不必要地拒收。实验室中进行的常规霉菌毒素分析仍基于不断改进的物理化学方法。霉菌毒素分析的进一步研究已在诸如薄层色谱法（TLC）、气相色谱法（GC）、高效液相色谱法（HPLC）和液相色谱-质谱联用（LC-MS）等筛选方法等技术方面展开。在霉菌毒素方法开发的某些领域，免疫亲和柱和多功能柱作为净化方法是不错的选择。它们适合替代传统的液-液分配或柱色谱净化方法。另一方面，对于进出口产品需要快速做出是与否的判定，这催生了一些新的筛选方法，主要是基于免疫分析原理的快速且易于使用的检测试剂盒。鉴于检测霉菌毒素的分析方法已变得更加普遍、灵敏和特异，对食品中霉菌毒素污染的监测也变得更为常见。方法的可靠性和明确的性能特征对于方法验证至关重要。本文涵盖了霉菌毒素定性和定量分析的一些最新活动及进展。
