Reshadi Samrand, Daie Ghazvini Roshanak, Hashemi Seyed Jamal, Bakhshi Heydar, Ardi Pegah, Shakibamehr Nafiseh, Babaie Mahdi, Dashtipour Shojaat, Rafat Zahra
Razi Vaccine and Serum Research Institute, Karaj, Iran.
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Sci Rep. 2025 May 29;15(1):18854. doi: 10.1038/s41598-025-87817-8.
As a highly fundamental crop with a strategic role in human and animal nutrition, corn is frequently contaminated with mycotoxins. Mycotoxin contamination of corn poses substantial risks to human health. This study aimed to evaluate the concentration of aflatoxins levels in three popular brands of cheese puffs prepared from corn flour. This cross-sectional study was carried out on 27 specimens of cheese puffs produced by three famous brands. Aflatoxins were extracted from dry specimens with methyl alcohol. The obtained leachate was diluted with distilled water and subsequently passed through an HPLC column containing antibodies specific to B- and G-group aflatoxins. Finally, the obtained data were analyzed statistically using t-test and chi-squared test. The highest concentration of aflatoxins in brands A, B, and C was observed in specimens A1 (1.35 ng/mL), B2 (0.95 ng/mL), and C2 (1.22 ng/mL), respectively. Additionally, specimens A3 (0.93 ng/mL), B3 (0.80 ng/mL), and C1 (0.75 ng/mL) contained the lowest levels of aflatoxins among these brands, respectively. The results indicated that the concentration of aflatoxins was lower than the permissible limit in Iran (5 µg/kg) in all understudied specimens.
作为一种在人类和动物营养中具有战略作用的重要基础作物，玉米经常受到霉菌毒素的污染。玉米中的霉菌毒素污染对人类健康构成重大风险。本研究旨在评估由玉米粉制成的三个流行品牌奶酪泡芙中黄曲霉毒素的浓度水平。这项横断面研究针对三个著名品牌生产的27个奶酪泡芙样本进行。用甲醇从干燥样本中提取黄曲霉毒素。所得浸出液用蒸馏水稀释，随后通过含有针对B族和G族黄曲霉毒素的特异性抗体的HPLC柱。最后，使用t检验和卡方检验对所得数据进行统计分析。品牌A、B和C中黄曲霉毒素的最高浓度分别在样本A1（1.35纳克/毫升）、B2（0.95纳克/毫升）和C2（1.22纳克/毫升）中观察到。此外，样本A3（0.93纳克/毫升）、B3（0.80纳克/毫升）和C1（0.75纳克/毫升）在这些品牌中分别含有最低水平的黄曲霉毒素。结果表明，在所有研究的样本中，黄曲霉毒素的浓度低于伊朗的允许限量（5微克/千克）。
