Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
College of Basic Medicine, Chongqing Medical University, Chongqing, China.
Toxicol Mech Methods. 2021 Jun;31(5):349-358. doi: 10.1080/15376516.2021.1876800. Epub 2021 Feb 9.
Smokeless tobacco products provide an alternative to cigarettes; however, smokeless tobacco is carcinogenic and harmful to human health. This study evaluated the toxicological effects of snus extracts and cigarette smoke total particulate matter (TPM) on human umbilical vein endothelial cells (HUVECs). Treated cells were examined for cell viability, reactive oxygen species (ROS), apoptosis, and inflammatory cytokines. Moreover, we explored the mechanism of programmed cell death induced by snus. The results showed that snus extracts significantly inhibited cell viability in a dose-dependent manner. ROS was significantly increased in treatment groups, and anti-oxidant treatment could not prevent snus extract-induced cell death. Snus extracts induced apoptosis, DNA damage, activation and cleavage of caspase-3 and caspase-8, pathway-related gene change, and interleukin (IL)-6 and IL-8 release in HUVECs. Snus extracts exposure may induce cytotoxicity, ROS generation, inflammatory cytokines release, and apoptosis or DNA damage through intrinsic and extrinsic pathways in HUVECs.
无烟烟草制品是香烟的替代品；然而，无烟烟草是致癌的，对人类健康有害。本研究评估了鼻烟提取物和香烟烟雾总颗粒物 (TPM) 对人脐静脉内皮细胞 (HUVEC) 的毒理学影响。用处理过的细胞检测细胞活力、活性氧 (ROS)、细胞凋亡和炎症细胞因子。此外，我们还探讨了 snus 诱导程序性细胞死亡的机制。结果表明，snus 提取物在一定浓度范围内显著抑制细胞活力，呈剂量依赖性。ROS 在治疗组中显著增加，抗氧化处理不能防止 snus 提取物诱导的细胞死亡。snus 提取物诱导细胞凋亡、DNA 损伤、caspase-3 和 caspase-8 的激活和切割、途径相关基因的改变，以及 HUVECs 中白细胞介素 (IL)-6 和 IL-8 的释放。snus 提取物暴露可能通过内在和外在途径在 HUVECs 中诱导细胞毒性、ROS 生成、炎症细胞因子释放以及细胞凋亡或 DNA 损伤。
