唾液病毒载量与儿童 2019 冠状病毒病的临床和免疫特征相关性更好。
Saliva viral load better correlates with clinical and immunological profiles in children with coronavirus disease 2019.
机构信息
Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, People's Republic of China.
Paediatric Infectious Disease Unit, Department of Paediatrics and Adolescent Medicine, Princess Margaret Hospital, Hong Kong SAR, People's Republic of China.
出版信息
Emerg Microbes Infect. 2021 Dec;10(1):235-241. doi: 10.1080/22221751.2021.1878937.
BACKGROUND
Pediatric COVID-19 studies exploring the relationships between NPS and saliva viral loads, clinical and immunological profiles are lacking.
METHODS
Demographics, immunological profiles, nasopharyngeal swab (NPS), and saliva samples collected on admission, and hospital length of stay (LOS) were assessed in children below 18 years with COVID-19.
FINDINGS
91 patients were included between March and August 20 20. NPS and saliva viral loads were correlated ( = 0.315, = 0.01). Symptomatic patients had significantly higher NPS and saliva viral loads than asymptomatic patients. Serial NPS and saliva viral load measurements showed that the log NPS ( = -0.532, < 0.001) and saliva ( = -0.417, < 0.001) viral loads for all patients were inversely correlated with the days from symptom onset with statistical significance. Patients with cough, sputum, and headache had significantly higher saliva, but not NPS, viral loads. Higher saliva, but not NPS, viral loads were associated with total lymphopenia, CD3 and CD4 lymphopenia (all < 0.05), and were inversely correlated with total lymphocyte ( = -0.43), CD3 ( = -0.55), CD4 ( = -0.60), CD8 ( = -0.41), B ( = -0.482), and NK ( = -0.416) lymphocyte counts (all < 0.05).
INTERPRETATION
Saliva viral loads on admission in children correlated better with clinical and immunological profiles than NPS.
背景
缺乏探索儿童 COVID-19 中 NPS 与唾液病毒载量、临床和免疫特征之间关系的儿科研究。
方法
评估了 2020 年 3 月至 8 月期间 18 岁以下 COVID-19 患儿的人口统计学特征、免疫特征、鼻咽拭子（NPS）和入院时采集的唾液样本以及住院时间（LOS）。
结果
共纳入 91 例患者。NPS 和唾液病毒载量相关（ = 0.315， = 0.01）。有症状的患者的 NPS 和唾液病毒载量明显高于无症状患者。连续的 NPS 和唾液病毒载量测量显示，所有患者的 NPS 对数（ = -0.532， < 0.001）和唾液（ = -0.417， < 0.001）病毒载量与从症状出现到测量日数呈负相关，具有统计学意义。有咳嗽、咳痰和头痛的患者的唾液病毒载量明显较高，但 NPS 病毒载量无明显升高。较高的唾液（但不是 NPS）病毒载量与总淋巴细胞减少、CD3 和 CD4 淋巴细胞减少有关（均 < 0.05），并与总淋巴细胞（ = -0.43）、CD3（ = -0.55）、CD4（ = -0.60）、CD8（ = -0.41）、B（ = -0.482）和 NK（ = -0.416）淋巴细胞计数呈负相关（均 < 0.05）。
结论
入院时的唾液病毒载量与临床和免疫特征相关性优于 NPS。
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