Minehart Jacqueline A, Speer Colenso M
Department of Biology, University of Maryland, College Park, MD, United States.
Front Synaptic Neurosci. 2021 Jan 5;12:615059. doi: 10.3389/fnsyn.2020.615059. eCollection 2020.
A key challenge in developmental neuroscience is identifying the local regulatory mechanisms that control neurite and synaptic refinement over large brain volumes. Innovative molecular techniques and high-resolution imaging tools are beginning to reshape our view of how local protein translation in subcellular compartments drives axonal, dendritic, and synaptic development and plasticity. Here we review recent progress in three areas of neurite and synaptic study -compartment-specific transcriptomics/translatomics, targeted proteomics, and super-resolution imaging analysis of synaptic organization and development. We discuss synergies between sequencing and imaging techniques for the discovery and validation of local molecular signaling mechanisms regulating synaptic development, plasticity, and maintenance in circuits.
发育神经科学中的一个关键挑战是确定控制大脑大区域内神经突和突触精细化的局部调节机制。创新的分子技术和高分辨率成像工具正开始重塑我们对于亚细胞区室中的局部蛋白质翻译如何驱动轴突、树突以及突触发育和可塑性的看法。在此，我们综述神经突和突触研究三个领域的最新进展——区室特异性转录组学/翻译组学、靶向蛋白质组学以及突触组织和发育的超分辨率成像分析。我们讨论测序和成像技术之间的协同作用，以发现和验证调节回路中突触发育、可塑性和维持的局部分子信号机制。
