Considerable increase in Poly(3-hydroxybutyrate) production via C gene overexpression in PTCC 1615.

Poly(3-hydroxybutyrate) (PHB) is a well-known biodegradable polymer produced by some microorganisms and can be a suitable alternative for petrochemical plastics. PHB synthase encoded by C gene is the main enzyme in PHB biosynthesis pathway in The aim of current study was the transformation of PTCC 1615 with its own C gene and evaluation of the overexpression effect on PHB accumulation. DNA fragment including phbC gene and its promoter and terminator regions, was isolated from PTCC 1615, inserted into pET28a(+) vector, and transferred to the competent bacteria using calcium chloride and heat shock method. The effect of the cloned gene expression on PHB production was investigated with absorption of crotonic acid produced through PHB dehydration. Statistical analyses were carried out by SPSS software. PHB content of cells of the engineered strain was 1.4 times more than that of the native bacteria. This significant difference can be an important finding for improvement of biopolymer production. Overexpression of C, the critical gene in PHB biosynthesis pathway, in PTCC 1615 had considerable effect on PHB accumulation.