洋葱伯克霍尔德菌H111菌株中σ54依赖的氮限制应答与毒力
σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111.
作者信息
Lardi Martina, Aguilar Claudio, Pedrioli Alessandro, Omasits Ulrich, Suppiger Angela, Cárcamo-Oyarce Gerardo, Schmid Nadine, Ahrens Christian H, Eberl Leo, Pessi Gabriella
机构信息
Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
出版信息
Appl Environ Microbiol. 2015 Jun 15;81(12):4077-89. doi: 10.1128/AEM.00694-15. Epub 2015 Apr 3.
Abstract
Members of the genus Burkholderia are versatile bacteria capable of colonizing highly diverse environmental niches. In this study, we investigated the global response of the opportunistic pathogen Burkholderia cenocepacia H111 to nitrogen limitation at the transcript and protein expression levels. In addition to a classical response to nitrogen starvation, including the activation of glutamine synthetase, PII proteins, and the two-component regulatory system NtrBC, B. cenocepacia H111 also upregulated polyhydroxybutyrate (PHB) accumulation and exopolysaccharide (EPS) production in response to nitrogen shortage. A search for consensus sequences in promoter regions of nitrogen-responsive genes identified a σ(54) consensus sequence. The mapping of the σ(54) regulon as well as the characterization of a σ(54) mutant suggests an important role of σ(54) not only in control of nitrogen metabolism but also in the virulence of this organism.
摘要
伯克霍尔德氏菌属的成员是多功能细菌，能够在高度多样的环境生态位中定殖。在本研究中，我们在转录和蛋白质表达水平上研究了机会致病菌洋葱伯克霍尔德菌H111对氮限制的全局反应。除了对氮饥饿的经典反应，包括谷氨酰胺合成酶、PII蛋白和双组分调节系统NtrBC的激活外，洋葱伯克霍尔德菌H111还响应氮短缺而上调了聚羟基丁酸酯（PHB）的积累和胞外多糖（EPS）的产生。在氮响应基因的启动子区域搜索共有序列，鉴定出一个σ(54)共有序列。σ(54)调控子的图谱绘制以及σ(54)突变体的表征表明，σ(54)不仅在氮代谢控制中起重要作用，而且在该生物体的毒力中也起重要作用。
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