• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation.

作者信息

Wright Mitchell Henry, Adelskov Joseph, Greene Anthony Carlson

机构信息

Division of Environmental and Biomolecular Systems, Institute of Environmental Health, Oregon Health & Science University, Portland, OR 97239-3098.

School of Natural Sciences, Griffith University, Nathan Campus, Queensland, Australia.

出版信息

J Microbiol Biol Educ. 2017 Sep 1;18(2). doi: 10.1128/jmbe.v18i2.1348. eCollection 2017.

DOI:10.1128/jmbe.v18i2.1348
PMID:28861145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5577976/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecba/5577976/a255535bff57/jmbe-18-48f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecba/5577976/a255535bff57/jmbe-18-48f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecba/5577976/a255535bff57/jmbe-18-48f1.jpg

相似文献

1
Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation.使用单一酶和苯酚/氯仿分离法提取细菌DNA
J Microbiol Biol Educ. 2017 Sep 1;18(2). doi: 10.1128/jmbe.v18i2.1348. eCollection 2017.
2
Comparison of a modified phenol/chloroform and commercial-kit methods for extracting DNA from horse fecal material.改良苯酚/氯仿法与商业试剂盒法从马粪便样本中提取DNA的比较。
J Microbiol Methods. 2016 Oct;129:14-19. doi: 10.1016/j.mimet.2016.07.019. Epub 2016 Jul 25.
3
[Comparing and evaluating six methods of extracting human genomic DNA from whole blood].[比较和评估从全血中提取人类基因组DNA的六种方法]
Fa Yi Xue Za Zhi. 2009 Apr;25(2):109-11, 114.
4
Evaluation of three extraction methods for molecular detection of Schistosoma mansoni infection in human urine and serum samples.用于分子检测人尿液和血清样本中曼氏血吸虫感染的三种提取方法的评估
J Parasit Dis. 2015 Sep;39(3):499-507. doi: 10.1007/s12639-013-0385-3. Epub 2013 Nov 20.
5
[Genomic DNA extraction from hair sacs of pigs using modified phenol-chloroform method].[采用改良酚氯仿法从猪毛囊中提取基因组DNA]
Yi Chuan. 2010 Jul;32(7):752-6. doi: 10.3724/sp.j.1005.2010.00752.
6
Optimization of phenol-chloroform RNA extraction.苯酚-氯仿RNA提取的优化
MethodsX. 2018 May 30;5:599-608. doi: 10.1016/j.mex.2018.05.011. eCollection 2018.
7
Standardization of DNA extraction from sand flies: Application to genotyping by next generation sequencing.白蛉DNA提取的标准化:在下一代测序基因分型中的应用
Exp Parasitol. 2017 Jun;177:66-72. doi: 10.1016/j.exppara.2017.04.010. Epub 2017 Apr 26.
8
Qualification study of two genomic DNA extraction methods in different clinical samples.两种基因组DNA提取方法在不同临床样本中的适用性研究
Tanaffos. 2014;13(4):41-7.
9
Genomic DNA extraction from whole blood stored from 15- to 30-years at -20 °C by rapid phenol-chloroform protocol: a useful tool for genetic epidemiology studies.快速酚-氯仿法从 -20°C 保存 15-30 年的全血中提取基因组 DNA:遗传流行病学研究的有用工具。
Mol Cell Probes. 2011 Feb;25(1):44-8. doi: 10.1016/j.mcp.2010.10.003. Epub 2010 Oct 26.
10
Evaluation of extraction methods from paraffin wax embedded tissues for PCR amplification of human and viral DNA.用于人及病毒DNA的PCR扩增的石蜡包埋组织提取方法的评估
J Clin Pathol. 2001 May;54(5):401-3. doi: 10.1136/jcp.54.5.401.

引用本文的文献

1
Draft genome sequence of HKEH-1 isolated from a diabetic patient's blood.从一名糖尿病患者血液中分离出的HKEH-1的基因组序列草图。
Microbiol Resour Announc. 2025 Sep 11;14(9):e0045225. doi: 10.1128/mra.00452-25. Epub 2025 Aug 11.
2
Plague and tularemia surveillance in hormozgan province, Southern Iran.伊朗南部霍尔木兹甘省的鼠疫和土拉菌病监测。
BMC Vet Res. 2025 Jul 2;21(1):428. doi: 10.1186/s12917-025-04884-9.
3
Complete genome sequence of pv. pathotype strain Pgu1, a phytopathogenic bacterium isolated from (geranium aralia).

本文引用的文献

1
A molecular phylogenetic framework for Bacillus subtilis using genome sequences and its application to Bacillus subtilis subspecies stecoris strain D7XPN1, an isolate from a commercial food-waste degrading bioreactor.利用基因组序列构建枯草芽孢杆菌的分子系统发育框架及其在枯草芽孢杆菌斯氏亚种菌株D7XPN1中的应用,该菌株分离自一个商业食品废弃物降解生物反应器。
3 Biotech. 2016 Jun;6(1):96. doi: 10.1007/s13205-016-0408-8. Epub 2016 Mar 28.
2
Production of Manganese Oxide Nanoparticles by Shewanella Species.希瓦氏菌属生产氧化锰纳米颗粒
Appl Environ Microbiol. 2016 Aug 15;82(17):5402-9. doi: 10.1128/AEM.00663-16. Print 2016 Sep 1.
3
从(龙芽楤木)分离得到的植物病原细菌pv.致病型菌株Pgu1的全基因组序列
Microbiol Resour Announc. 2025 Jul 10;14(7):e0024025. doi: 10.1128/mra.00240-25. Epub 2025 Jun 17.
4
Isolation of a strain and identification of methyltransferase genes conferring the high arsenic volatilizing ability.具有高砷挥发能力的菌株分离及甲基转移酶基因鉴定
Appl Environ Microbiol. 2025 Jun 18;91(6):e0246724. doi: 10.1128/aem.02467-24. Epub 2025 May 30.
5
Constituent of extracellular polymeric substances (EPS) produced by a range of soil bacteria and fungi.一系列土壤细菌和真菌产生的细胞外聚合物(EPS)的组成成分。
BMC Microbiol. 2025 May 15;25(1):298. doi: 10.1186/s12866-025-04034-z.
6
Screening, Identification, and Optimization of Protease Producing Strain DT-15 From Tannery Waste Disposal Site in Addis Ababa, Ethiopia.埃塞俄比亚亚的斯亚贝巴制革厂废物处理场产蛋白酶菌株DT-15的筛选、鉴定及优化
Int J Microbiol. 2025 Apr 29;2025:7176092. doi: 10.1155/ijm/7176092. eCollection 2025.
7
Characterization and optimization of azo dyes degrading microbes isolated from textile effluent.从纺织废水中分离出的偶氮染料降解微生物的特性及优化
Sci Rep. 2025 Apr 2;15(1):11241. doi: 10.1038/s41598-025-95359-2.
8
Exploring caffeine as a disruptor of membrane integrity and genomic stability in Staphylococcus aureus: functional and in silico analysis.探索咖啡因作为金黄色葡萄球菌膜完整性和基因组稳定性的破坏剂:功能分析和计算机模拟分析
Arch Microbiol. 2025 Jan 8;207(2):28. doi: 10.1007/s00203-024-04230-x.
9
Complete genome sequence of ES191 generated by PacBio sequencing.通过PacBio测序生成的ES191的完整基因组序列。
Microbiol Resour Announc. 2024 Dec 12;13(12):e0047324. doi: 10.1128/mra.00473-24. Epub 2024 Nov 8.
10
Genomic and phenotypic characterization of isolates from two Mexican cystic fibrosis attention centers.来自两个墨西哥囊性纤维化治疗中心的分离株的基因组和表型特征
Microbiol Spectr. 2024 Oct 23;12(12):e0110024. doi: 10.1128/spectrum.01100-24.
Impacts of gut bacteria on human health and diseases.
肠道细菌对人类健康和疾病的影响。
Int J Mol Sci. 2015 Apr 2;16(4):7493-519. doi: 10.3390/ijms16047493.
4
Biosafety guidelines for handling microorganisms in the teaching laboratory: development and rationale.教学实验室中微生物处理的生物安全指南:制定与基本原理
J Microbiol Biol Educ. 2013 May 6;14(1):78-83. doi: 10.1128/jmbe.v14i1.531. Print 2013.
5
Caloramator australicus sp. nov., a thermophilic, anaerobic bacterium from the Great Artesian Basin of Australia.澳大利亚热厌氧杆菌新种,一种来自澳大利亚大自流盆地的嗜热厌氧菌。
Int J Syst Evol Microbiol. 2009 Jan;59(Pt 1):95-101. doi: 10.1099/ijs.0.000802-0.
6
Comparison of three DNA extraction methods for Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium.牛分枝杆菌、结核分枝杆菌和鸟分枝杆菌鸟亚种三种DNA提取方法的比较
Lett Appl Microbiol. 2008 Jul;47(1):8-11. doi: 10.1111/j.1472-765X.2008.02372.x. Epub 2008 May 21.