CNRS, Aix-Marseille Université, Laboratoire de Bioénergétique et Ingénierie des Protéines, Marseille, France.
Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Universitätsstr. 150, Bochum D-44780, Germany.
Chem Commun (Camb). 2021 Feb 19;57(14):1750-1753. doi: 10.1039/d0cc08168j.
We demonstrate that the insertion of the dinuclear active site of [FeFe] hydrogenase into the apo-enzyme can occur when the enzyme is embedded in a film of redox polymer, under conditions of mediated electron transfer. The maturation can be monitored by electrochemistry, and is as fast as under conditions of direct electron transfer. This new approach further clears the way to the implementation of hydrogenases in large scale real life processes.
我们证明，在介导电子转移的条件下，当酶嵌入氧化还原聚合物薄膜中时，[FeFe]氢化酶的双核活性位点可以插入脱辅基酶中。成熟过程可以通过电化学监测，并且与直接电子转移条件下一样快。这种新方法进一步为在大规模实际生活过程中实施氢化酶铺平了道路。
