Santos Carvalhais Bárbara Ellen, Souza E Silva Cristiana de, Dos Santos Kênia Valéria
Department of Pathology, Health Sciences Center, Federal University of Espírito Santo (UFES), Av. Marechal Campos, Vitória, Espírito Santo 1468 29040 090, Brazil.
Future Microbiol. 2021 Jan;16(2):83-93. doi: 10.2217/fmb-2020-0115. Epub 2021 Jan 20.
To evaluate the activity of five antimicrobials against young and mature biofilms. Nineteen clinical strains from hemoculture of hemodialysis patients were tested for biofilm kinetics, MIC and minimum biofilm inhibitory concentration (MBIC) in young and mature biofilms. All strains were moderate biofilm producers. MIC showed total susceptibility to levofloxacin and trimethoprim-sulfamethoxazole and partial resistance to ceftazidime (63.2%) and gentamicin (21%). Young and mature biofilms showed the lowest MBIC/MIC ratio for gentamicin, chloramphenicol and levofloxacin, respectively. The highest MBIC/MIC was for trimethoprim-sulfamethoxazole (young) and ceftazidime (mature). Gentamicin displayed surprising activity against biofilms. Chloramphenicol was indicated as a good option against young biofilms, and trimethoprim-sulfamethoxazole showed limited antibiofilm activity.
为评估五种抗菌药物对年轻和成熟生物膜的活性。对19株来自血液透析患者血培养的临床菌株进行了生物膜动力学、最低抑菌浓度（MIC）以及年轻和成熟生物膜中的最低生物膜抑制浓度（MBIC）检测。所有菌株均为中度生物膜形成菌。MIC结果显示对左氧氟沙星和复方新诺明完全敏感，对头孢他啶（63.2%）和庆大霉素（21%）部分耐药。年轻和成熟生物膜分别对庆大霉素、氯霉素和左氧氟沙星显示出最低的MBIC/MIC比值。MBIC/MIC最高的是复方新诺明（年轻生物膜）和头孢他啶（成熟生物膜）。庆大霉素对生物膜表现出惊人的活性。氯霉素被认为是对抗年轻生物膜的良好选择，而复方新诺明的抗生物膜活性有限。
