Department of Restorative Dentistry, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, São Paulo, 14040-904, Brazil.
Department of Chemistry, Center of Nanotechnology and Tissue Engineering, Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, 14040-901, Brazil.
J Mater Sci Mater Med. 2021 Jan 20;32(1):11. doi: 10.1007/s10856-020-06487-2.
To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment. The sample consisted of 22 patients (age between 33 and 52 years) with shallow or medium erosion lesions located in two homologous teeth. The teeth were randomly assigned according to dentin treatment: with 2.5% NanoChi and without with chitosan (control). The NanoChi were applied immediately after acid etching. The teeth were restored with Single Bond Universal (3 M) and Charisma resin (Kulzer). Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year. Population demographics, Kaplan-Meier estimates and log-rank test (Mantel-Cox) were calculated for 1 year (α = 0.05). No significant difference was found in the survival rates between groups (p > 0.05) at 7 days and 1 year after treatment. After 7 days, 100% of the restorations were scored as Alpha on all criteria. After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie. Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations. The biomodification of eroded dentin with 2.5% NanoChi did not influence the survival of the restorations after 1 year. The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.
评价 2.5%纳米壳聚糖（2.5% NanoChi）溶液预处理对修复牙科治疗前牙本质侵蚀的影响。该样本由 22 名（年龄 33 至 52 岁）患有浅或中度侵蚀病变的患者组成，病变位于两颗同源牙上。牙齿根据牙本质处理随机分配：使用 2.5% NanoChi 和不使用壳聚糖（对照组）。纳米壳聚糖在酸蚀后立即应用。牙齿用 Single Bond Universal（3M）和 Charisma 树脂（Kulzer）修复。在 7 天（基线）和 1 年内，使用改良 USPHS（保留、继发龋、边缘适应性和敏感性）和摄影（颜色、边缘色素沉着和解剖形态）标准进行分析。在 1 年内计算人口统计学、Kaplan-Meier 估计和对数秩检验（Mantel-Cox）（α=0.05）。在治疗后 7 天和 1 年内，两组的生存率无显著差异（p>0.05）。在 7 天后，所有标准的所有修复体均得分为 Alpha。在 1 年后，91%的 NanoChi 修复体的保留、边缘适应性和解剖形态标准得分为 Alpha，9%得分为 Charlie，而 9%的对照组（无 NanoChi）修复体得分为 Alpha，14%得分为 Charlie。继发龋、敏感性、颜色和边缘色素沉着标准的 100%修复体得分为 Alpha。用 2.5% NanoChi 对牙本质进行生物修饰后，1 年后修复体的存活率没有受到影响。在 1 年内，纳米壳聚糖处理牙本质不会增加树脂修复体的失败，因此可以用作预处理溶液。
