载姜黄素的不同包衣聚合物纳米胶囊在鸡胚模型中的评价：对血管生成、致畸和氧化应激的影响。
Evaluation of curcumin-loaded polymeric nanocapsules with different coatings in chick embryo model: influence on angiogenesis, teratogenesis and oxidative stress.
机构信息
Postgraduate Program in Pharmaceutical Sciences, Federal University of Pampa, Campus Uruguaiana, BR 472, Km 7, Uruguaiana, RS, 97500-970, Brazil.
Pharmacy Course, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil.
出版信息
Pharmacol Rep. 2021 Apr;73(2):563-573. doi: 10.1007/s43440-021-00218-2. Epub 2021 Jan 20.
BACKGROUND
Curcumin (CUR) is a bioactive compound with several proven pharmacological properties. However, the major limitation for therapeutic use of CUR is its low bioavailability. In this sense, an alternative to this question is the use of polymeric nanocapsules (NC) as drug/nutraceutical delivery systems. Thus, the aim of current study was to assess the effect of CUR-loaded NC and their different coatings in chick embryo model, evaluating angiogenic, teratogenic and oxidative stress parameters.
METHODS
The physicochemical characterization of unloaded and loaded NC with different coatings: (U-NC (P80), U-NC (PEG), U-NC (EUD), U-NC (CS), CUR-NC (P80), CUR-NC (PEG), CUR-NC (EUD) and CUR-NC (CS)) were performed. After 9 days of incubation, eggs were treated (10 mL/kg eggs; via injection) with NC (unloaded and loaded with CUR) and CUR-solution. In sequence, hen's egg test-chorioallantoic membrane (HET-CAM), angiogenic assay, external abnormalities, weight of embryos and oxidative stress markers (TBARS, NPSH, ROS and CAT) were analyzed.
RESULTS
CUR-NC (P80, PEG, EUD and CS) treatments caused antiangiogenic and non-teratogenic effects in chick embryo model. Still, CUR-NC (P80), CUR-NC (PEG), CUR-NC (EUD) and CUR-NC (CS) did not alter markers of oxidative stress (TBARS, NPSH, CAT) studied. Only CUR-NC (EUD) caused increase in ROS levels.
CONCLUSION
Wherefore, these findings of present study represent a advance in research of drug/nutraceutical delivery systems.
背景
姜黄素（CUR）是一种具有多种已证实的药理学特性的生物活性化合物。然而，CUR 治疗用途的主要限制是其生物利用度低。在这种情况下，CUR 替代物是使用聚合物纳米胶囊（NC）作为药物/营养保健品的递送系统。因此，目前研究的目的是评估负载 CUR 的 NC 及其不同涂层对鸡胚模型的影响，评估血管生成、致畸和氧化应激参数。
方法
对未负载和负载不同涂层的 NC（U-NC（P80）、U-NC（PEG）、U-NC（EUD）、U-NC（CS）、CUR-NC（P80）、CUR-NC（PEG）、CUR-NC（EUD）和 CUR-NC（CS））进行理化特性表征。孵育 9 天后，用 NC（未负载和负载 CUR）和 CUR 溶液处理鸡蛋。随后，进行鸡胚绒毛尿囊膜（HET-CAM）试验、血管生成试验、外部异常、胚胎重量和氧化应激标志物（TBARS、NPSH、ROS 和 CAT）分析。
结果
CUR-NC（P80、PEG、EUD 和 CS）处理在鸡胚模型中引起抗血管生成和非致畸作用。然而，CUR-NC（P80）、CUR-NC（PEG）、CUR-NC（EUD）和 CUR-NC（CS）并未改变所研究的氧化应激标志物（TBARS、NPSH、CAT）。只有 CUR-NC（EUD）引起 ROS 水平升高。
结论
因此，本研究的这些发现代表了药物/营养保健品递送系统研究的进展。
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