Mother & Child Health, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed to be) University, Pune 411043, India.
Genomic Research on Complex diseases (GRC Group), Council of Scientific and Industrial Research Centre for Cellular & Molecular Biology (CSIR-CCMB), Hyderabad 500007, India.
Epigenomics. 2021 Feb;13(4):257-269. doi: 10.2217/epi-2020-0318. Epub 2021 Jan 20.
This study aims to examine the DNA methylation (DNAm) and expression patterns of genes associated with placental angiogenesis in preeclampsia. DNAm and expression were examined in normotensive (n = 100) and preeclampsia (n = 100) women using pyrosequencing and quantitative real-time PCR respectively. Hypomethylation at several CpGs was observed in and in women with preeclampsia compared to normotensive controls. expression was lower in women with preeclampsia while expression was comparable. DNAm at various CpGs was negatively correlated with expression in both the genes and were associated with maternal blood pressure and birth outcomes. DNAm and expression of angiogenic factors in placentae are differentially regulated in preeclampsia and influence birth outcomes.
本研究旨在探讨与子痫前期胎盘血管生成相关的基因的 DNA 甲基化 (DNAm) 和表达模式。 分别使用焦磷酸测序和定量实时 PCR 检测正常妊娠（n=100）和子痫前期（n=100）妇女的 DNAm 和表达。 与正常妊娠对照组相比，子痫前期妇女的 和 中存在多个 CpG 低甲基化。 子痫前期妇女的 表达较低，而 表达相当。 在两个基因中，DNAm 与表达呈负相关，并且与母亲血压和出生结局相关。 子痫前期胎盘血管生成因子的 DNAm 和表达存在差异调节，并影响出生结局。
